Aizawa S, Kurimoto F
Mech Ageing Dev. 1979 Nov;11(4):237-43. doi: 10.1016/0047-6374(79)90003-4.
Concanavalin A (Con A)-mediated red blood cell (RBC) adsorption with the RBC coating method (in which Con A-coated RBC's are adsorbed to fibroblasts) was greatly increased by glutaraldehyde fixation of RBCs before Con A-coating and decreased by the fixation of fibroblasts. On the other hand, RBC adsorption with the fibroblast coating method (in which RBCs are adsorbed to Con A-coated fibroblasts) was decreased by the fixation of RBCs and increased by the fixation of fibroblasts before Con A coating. The fixation of RBCs or fibroblasts after Con A coating did not have these effects. In addition, the fixation of both RBCs and fibroblasts nearly completely abolished RBC adsorption with either method. However, the amount of [3H] Con A binding was not affected by the fixation. RBC adsorption with the fibroblast coating method was also affected by cytochalasin B, colchicine, NaN3 and dibucane treatments of fibroblasts. These drug treatments of fibroblasts, however, did not affect RBC adsorption with the RBC coating method, except cytochalasin B. In addition, the effects of drug treatments of fibroblasts examined occurred nearly to the same extent for nonsenescent, senescent, and transformed cells. Our results suggest that secondary processes after Con A binding, receptor mobility and receptor association with cytoskeletals, play important roles in RBC adsorption, but that the roles do not change with aging or transformation.
用红细胞包被法（将伴刀豆球蛋白A（Con A）包被的红细胞吸附到成纤维细胞上）进行的Con A介导的红细胞（RBC）吸附，在Con A包被前用戊二醛固定红细胞可使其显著增加，而成纤维细胞固定后则降低。另一方面，用成纤维细胞包被法（将红细胞吸附到Con A包被的成纤维细胞上）进行的RBC吸附，红细胞固定后降低，Con A包被前成纤维细胞固定则增加。Con A包被后红细胞或成纤维细胞的固定没有这些影响。此外，红细胞和成纤维细胞都固定几乎完全消除了两种方法的RBC吸附。然而，[3H]Con A结合量不受固定的影响。用成纤维细胞包被法进行的RBC吸附也受细胞松弛素B、秋水仙碱、NaN3和地布卡因对成纤维细胞处理的影响。然而，这些对成纤维细胞的药物处理，除细胞松弛素B外，不影响用红细胞包被法进行的RBC吸附。此外，所检测的对成纤维细胞药物处理的效果在未衰老、衰老和转化细胞中几乎以相同程度发生。我们的结果表明，Con A结合后的次级过程、受体移动性以及受体与细胞骨架的结合在RBC吸附中起重要作用，但这些作用不会随衰老或转化而改变。
