Aizawa S, Kurimoto F
Mech Ageing Dev. 1979 Nov;11(4):245-52. doi: 10.1016/0047-6374(79)90004-6.
Two types of age-related cell surface changes could be demonstrated in human diploid fibroblasts with the two methods of the lectin-mediated red blood cell (RBC) adsorption assay: the fibroblast coating method (in which RBCs are adsorbed to lectin-coated fibroblasts) and the RBC coating method (in which lectin-coated RBCs are adsorbed to fibroblasts). With the fibroblast coating method, concanavalin A and agglutinin L from Phaseolus vulgaris gave a change in RBC adsorption which did not occur throughout the phase II period, but increased with the advance of the phase III period (type I). With the RBC coating method, these lectins gave another type of change in RBC adsorption which increased continuously from early phases of cell passage up through cell senescence (type II). Ricinus communis agglutinin 120 also gave the type I change in RBC adsorption with the fibroblast coating method. On the other hand, even with the fibroblast coating method, Phaseolus vulgaris agglutinin E and wheat-germ agglutinin gave the type II change in RBC adsorption. Soy bean agglutinin and Bauhinia purpurea agglutinin gave only a restricted amount of RBC adsorption. Lens culinaris agglutin, pokeweed mitogen, Dolichos biflorus agglutinin, Lotus tetragonolobus agglutinin, Limulus polyhemus agglutinin and divalent succinylated concanavalin A did not give any RBC adsorption throughout the life span of human diploid fibroblasts.
采用凝集素介导的红细胞（RBC）吸附试验的两种方法，可以在人二倍体成纤维细胞中证实两种与年龄相关的细胞表面变化：成纤维细胞包被法（其中红细胞吸附到凝集素包被的成纤维细胞上）和红细胞包被法（其中凝集素包被的红细胞吸附到成纤维细胞上）。采用成纤维细胞包被法时，刀豆球蛋白A和菜豆凝集素L会引起红细胞吸附的变化，这种变化在整个II期都不会发生，但会随着III期的推进而增加（I型）。采用红细胞包被法时，这些凝集素会引起另一种红细胞吸附变化，这种变化从细胞传代早期一直持续增加直至细胞衰老（II型）。蓖麻凝集素120采用成纤维细胞包被法时也会引起I型红细胞吸附变化。另一方面，即使采用成纤维细胞包被法，菜豆凝集素E和麦胚凝集素也会引起II型红细胞吸附变化。大豆凝集素和紫羊蹄甲凝集素只能引起有限量的红细胞吸附。在人二倍体成纤维细胞的整个生命周期中，小扁豆凝集素、商陆有丝分裂原、双花扁豆凝集素、四棱豆凝集素、鲎凝集素和二价琥珀酰化刀豆球蛋白A都不会引起任何红细胞吸附。
