间接ELISA和夹心ELISA检测奶牛牛呼吸道合胞体病毒（BRSV）抗体的比较评估
Comparative evaluation of indirect and sandwich ELISA for the detection of antibodies to bovine respiratory syncytial virus (BRSV) in dairy cattle.
作者信息
Hazari S, Panda H K, Kar B C, Das B R
机构信息
Department of Bacteriology and Virology, College of Veterinary Science and Animal Husbandry, Orissa University of Agriculture and Technology, Orissa, India.
出版信息
Comp Immunol Microbiol Infect Dis. 2002 Jan;25(1):59-68. doi: 10.1016/s0147-9571(01)00022-4.
Seroprevalence of bovine respiratory syncytial virus (BRSV) infection in both exotic and crossbred cattle were described. A baculovirus expressed recombinant purified nucleocapsid (N) protein was used in indirect and sandwich ELISA for screening of 499 bovine sera samples from all over the state for the presence of BRSV antibodies. The seroprevalence rate of BRSV was found to be 46.09% through indirect ELISA while it would found to be 65.33% by sandwich ELISA. The result also indicated that exotic breeds were more susceptible to BRSV infection compared to crossbred cattle. A comprehensive analysis on susceptibility to BRSV as regards to various factors like age and sex was also summarized.
描述了外来牛和杂交牛中牛呼吸道合胞病毒（BRSV）感染的血清流行率。一种杆状病毒表达的重组纯化核衣壳（N）蛋白用于间接和夹心ELISA，以筛查来自该州各地的499份牛血清样本中是否存在BRSV抗体。通过间接ELISA发现BRSV的血清流行率为46.09%，而通过夹心ELISA发现为65.33%。结果还表明，与杂交牛相比，外来品种对BRSV感染更易感。还总结了关于年龄和性别等各种因素对BRSV易感性的综合分析。
Zotero 插件