Thomas T M, Shave E E, Bate I M, Gee S C, Franklin S, Rylatt D B
Gradipore Ltd., Frenchs Forest, NSW, Australia.
J Chromatogr A. 2002 Jan 25;944(1-2):161-8. doi: 10.1016/s0021-9673(01)01283-3.
Antibodies were purified from normal rabbit, sheep, goat, rat, human and bovine serum using preparative electrophoresis on a Gradiflow in a single-step process using an asymmetrical cartridge with three different pore size polyacrylamide membranes. Recoveries in each case were over 80% and were higher than those obtained using affinity chromatography on protein A, protein G or protein L. Degree of purity was at least comparable with these methods. These results suggest that preparative electrophoresis can be considered a general method for the purification of research quantities of antibodies from multiple serum sources and may be particularly useful where the reactivity with protein A, G or L is unknown.
使用带有三种不同孔径聚丙烯酰胺膜的不对称柱，通过在Gradiflow上进行制备电泳，从正常兔、羊、山羊、大鼠、人及牛血清中一步法纯化抗体。每种情况下的回收率均超过80%，且高于使用蛋白A、蛋白G或蛋白L亲和层析法获得的回收率。纯度至少与这些方法相当。这些结果表明，制备电泳可被视为从多种血清来源纯化研究用量抗体的通用方法，在与蛋白A、G或L的反应性未知的情况下可能特别有用。
