Horvath Z S, Gooley A A, Wrigley C W, Margolis J, Williams K L
Macquarie University Centre for Analytical Biotechnology, North Ryde, Australia.
Electrophoresis. 1996 Jan;17(1):224-6. doi: 10.1002/elps.1150170138.
Using the patented Gradiflow system in conjunction with newly developed affinity membranes, the suitability of an electrokinetic technique for affinity fractionation was investigated. Blue dextran incorporated into an affinity membrane was used to deplete a solution of horse serum of albumin, with the result that a majority of serum proteins were enriched tenfold relative to albumin. The technique, when fully developed, would offer some advantages over affinity chromatography, since to a degree it is possible to control which components of the sample are presented to the affinity matrix. Furthermore, the technique would extend the capabilities of the already multifunctional Gradiflow system.
结合新开发的亲和膜使用专利的Gradiflow系统，研究了一种电动技术用于亲和分级分离的适用性。掺入亲和膜中的蓝色葡聚糖用于去除马血清溶液中的白蛋白，结果是大多数血清蛋白相对于白蛋白富集了十倍。该技术充分发展后，将比亲和色谱法具有一些优势，因为在一定程度上可以控制样品的哪些组分与亲和基质接触。此外，该技术将扩展已经多功能的Gradiflow系统的功能。
