[三个牛品种的分子与遗传多态性]

[Molecular and genetic polymorphism in three cattle breeds].

作者信息

Zubets M V, Burkat V P, Sivolap Iu M, Kuznetsov V E, Lovenchuk I N

出版信息

Tsitol Genet. 2001 Jul-Aug;35(4):3-11.

Abstract

Determination of cattle breed and assignment of individual animals to that or another group based on distinctions of genotype is of great importance in genetics and selection. Using of PCR-analysis opened new possibilities for typing DNA. The procedure of PCR was optimized for animal DNA. Two methods of PCR, RAPD and SSRP, were applied for investigation of molecular-genetical polymorphism in three cattle breeds (Ukrainian red-motley lactescent, Golshtine and Simmental). A dendrogram reflecting genetical mutual relations between analyzed animals was drawn. The level of homo-heterozygosity and the distribution of microsatellite alleles were determined. System for investigation of molecular-genetical polymorphism in the cattle breeds and characterization of genotype of individual animals were developed and tested on a real population.

摘要

基于基因型差异来确定牛的品种并将个体动物划分到该品种或其他品种组在遗传学和选育中具有重要意义。PCR分析的应用为DNA分型开辟了新的可能性。PCR程序已针对动物DNA进行了优化。两种PCR方法，即随机扩增多态性DNA（RAPD）和简单序列重复多态性（SSRP），被用于研究三个牛品种（乌克兰红白花乳牛、戈尔什丁牛和西门塔尔牛）的分子遗传多态性。绘制了反映所分析动物之间遗传相互关系的树形图。确定了纯合-杂合水平以及微卫星等位基因的分布。开发了用于研究牛品种分子遗传多态性和个体动物基因型特征的系统，并在实际种群中进行了测试。