Erden-Inal Mine, Sunal Emine, Kanbak Güngör
Osmangazi University, The Medical School, Department of Biochemistry Eskişehir, Turkey.
Cell Biochem Funct. 2002 Mar;20(1):61-6. doi: 10.1002/cbf.937.
The effect of aging on the glutathione redox system was evaluated in this study. For this purpose, we determined reduced glutathione (GSH) and oxidized glutathione (GSSG) in whole blood, glutathione peroxidase (GPx) and glutathione reductase (GSSGR) in erythrocytes and selenium (Se) in plasma in 176 healthy individuals. We also calculated GSH/GSSG molar ratios. These subjects were divided into five groups: group 1 (n=25; 0.2-1 years old); group 2 (n=28; 2-11 years old); group 3 (n=23; 12-24 years old); group 4 (n=40; 25-40 years old); group 5 (n=60; 41-69 years old). GSH levels in groups 1 and 5 were significantly lower than the other groups (p<0.001). Conversely, GSSG levels were significantly high in these periods (p<0.001). The GSH/GSSG molar ratio was found to be low both in the first year of life and in the oldest group (p<0.001, respectively). GPx activity in group 5 was increased as compared to the other groups (p<0.001). GSSGR activity was significantly lower in the oldest groups than in the other groups (p<0.001). Se levels were found to be low in the oldest group (p<0.001). Selenium levels of women in group 5 were significantly high as compared to the men (p<0.01). We found negative correlations between age and GSH levels (r=0.402; p<0.001), selenium levels (r=0.454; p<0.001), GSH/GSSG molar ratio (r=0.557; p<0.001) and GSSGR activity (r=0.556; p<0.001). There were positive correlations between age and GPx (r=0.538; p<0.001) and GSSG level (r=0.551; p<0.001). In conclusion, our findings show that the glutathione redox system is affected by age. Oxidative stress increases during the aging process. There is no effect of aging on the glutathione redox system according to sex except for the Se level.
本研究评估了衰老对谷胱甘肽氧化还原系统的影响。为此，我们测定了176名健康个体全血中的还原型谷胱甘肽（GSH）和氧化型谷胱甘肽（GSSG）、红细胞中的谷胱甘肽过氧化物酶（GPx）和谷胱甘肽还原酶（GSSGR）以及血浆中的硒（Se）。我们还计算了GSH/GSSG摩尔比。这些受试者被分为五组：第1组（n = 25；0.2 - 1岁）；第2组（n = 28；2 - 11岁）；第3组（n = 23；12 - 24岁）；第4组（n = 40；25 - 40岁）；第5组（n = 60；41 - 69岁）。第1组和第5组的GSH水平显著低于其他组（p < 0.001）。相反，在这些时期GSSG水平显著升高（p < 0.001）。发现生命的第一年和最年长组的GSH/GSSG摩尔比均较低（分别为p < 0.001）。与其他组相比，第5组的GPx活性增加（p < 0.001）。最年长组的GSSGR活性显著低于其他组（p < 0.001）。发现最年长组的Se水平较低（p < 0.001）。第5组女性的硒水平显著高于男性（p < 0.01）。我们发现年龄与GSH水平（r = 0.402；p < 0.001）、硒水平（r = 0.454；p < 0.001）、GSH/GSSG摩尔比（r = 0.557；p < 0.001）和GSSGR活性（r = 0.556；p < 0.001）之间呈负相关。年龄与GPx（r = 0.538；p < 0.001）和GSSG水平（r = 0.551；p < 0.
