Ozoani Harrison A, Orisakwe Orish Ebere, Parisi Costantino, Assisi Loredana, Ezejiofor Anthonet N, Okolo Kenneth O, Orish Chinna N, Vangone Rubina, Sivieri Emidio M, Guerriero Giulia
Word Bank Africa Centre of Excellence in Oilfield Chemicals Research (ACE-CEFOR), University of Port Harcourt, PMB, Choba, Port Harcourt 5323, Nigeria.
Advanced Research Centre, European University of Lefke, Northern Cypus, Lefke, TR-10, Mersin 99101, Turkey.
Antioxidants (Basel). 2024 Aug 24;13(9):1028. doi: 10.3390/antiox13091028.
Male fertility is strongly affected by the overexpression of free radicals induced by heavy metals. The aim of this study was to evaluate the potential antioxidant, anti-inflammatory, and gonado-protective effects of natural compounds. Biochemical and morphological assays were performed on male albino rats divided into five groups: a control group (water only), a group orally exposed to a metal mixture of Pb-Cd-Hg-As alone and three groups co-administered the metal mixture and an aqueous extract of the Nigerian medicinal plant, (, ), at three different concentrations (500, 1000, and 1500 mg/kg) for 60 days. The metal mixture induced a significant rise in testicular weight, metal bioaccumulation, oxidative stress, and pro-inflammatory and apoptotic markers, while the semen analysis indicated a lower viability and a decrease in normal sperm count, and plasma reproductive hormones showed a significant variation. Parallel phytochemical investigations showed that has bioactive compounds like phlobatannins, flavonoids, polyphenols, tannins, saponins, steroids, and alkaloids, which are protective against oxidative injury in neural tissues. Indeed, the presence of co-administered with the metal mixture mitigated the toxic metals' impact, which was determined by observing the oxido-inflammatory response via nuclear factor erythroid 2-related factor 2, thus boosting male reproductive health.
重金属诱导的自由基过表达对男性生育能力有强烈影响。本研究的目的是评估天然化合物的潜在抗氧化、抗炎和性腺保护作用。对雄性白化大鼠进行生化和形态学分析，将其分为五组：对照组（仅给予水）、单独口服暴露于铅 - 镉 - 汞 - 砷金属混合物的组以及三组同时给予金属混合物和尼日利亚药用植物（此处植物名称缺失）水提取物的组，水提取物有三种不同浓度（500、1000和1500毫克/千克），持续60天。金属混合物导致睾丸重量显著增加、金属生物蓄积、氧化应激以及促炎和凋亡标志物升高，而精液分析表明活力降低且正常精子数量减少，血浆生殖激素也有显著变化。平行的植物化学研究表明（此处植物名称缺失）含有生物活性化合物，如间苯三酚鞣质、黄酮类、多酚类、单宁、皂苷、甾体和生物碱，这些对神经组织的氧化损伤具有保护作用。确实，与金属混合物同时给予（此处植物名称缺失）可减轻有毒金属的影响，这通过观察核因子红细胞2相关因子2的氧化炎症反应来确定，从而改善男性生殖健康。
