Hu Bo, Zhou Xin, Li Lin, Li Xi
Department of Clinical Laboratory, the Third Affiliated Hospital, Sun Yat-Sen University of Medical Sciences, Guangzhou, Guangdong, 510630 P. R. China.
Zhonghua Yi Xue Yi Chuan Xue Za Zhi. 2002 Feb;19(1):46-8.
This study inquired into the relationship between a pentanucleotide repeats(PNR) polymorphism of the apolipoprotein(a)[apo(a)] gene and coronary atherosclerotic heart disease(CHD) in Chinese Han nationality.
PNR polymorphism of the apo(a) gene from 165 cases of CHD and 153 normal individuals were analyzed by polymerase chain reaction(PCR)-denature polyacrylamide gel electrophoresis-silver stain.
The frequencies of (TTTTA)(5/8) genotype (0.188) and (TTTTA)(5) allele (0.115) in CHD group were remarkably higher than those in control group (0.039, 0.026)(P<0.01, P<0.05).
These findings indicate that the PNR polymorphism of apo(a) is associated with the susceptibility to CHD, which may be involved in the development of CHD.
本研究探讨载脂蛋白(a)[apo(a)]基因五核苷酸重复序列(PNR)多态性与中国汉族人群冠状动脉粥样硬化性心脏病(CHD)的关系。
采用聚合酶链反应(PCR)-变性聚丙烯酰胺凝胶电泳-银染法分析165例冠心病患者和153例正常个体的apo(a)基因PNR多态性。
冠心病组(TTTTA)(5/8)基因型频率(0.188)和(TTTTA)(5)等位基因频率(0.115)显著高于对照组(0.039, 0.026)(P<0.01, P<0.05)。
这些结果表明，apo(a)基因的PNR多态性与冠心病易感性相关，可能参与了冠心病的发生发展。
