Zeiger E, Chhabra R S, Margolin B H
Mutat Res. 1979 Dec;64(6):379-89. doi: 10.1016/0165-1161(79)90108-0.
The mutagenicity of benzo[alpha]pyrene and 2-aminoanthracene for Salmonella typhimurium TA98 in the plate-incorporation test was studied using liver S9 from untreated and aroclor-1254-treated rats. The induction of liver S9 protein, arylhydrocarbon hydroxylase (AHH), and cytochrome P448/450 was followed with time. There was no change in protein concentrations with induction; AHH and cytochrome levels were increased at 1, 3, 5 and 7 days post Aroclor treatment. Benzo[alpha]pyrene mutagenicity was enhanced with Aroclor treatment while 2-aminoanthracene mutagenicity was depressed. The benzo[alpha]pyrene mutagenicity showed a positive correlation with the levels of AHH and cytochrome on the plate; 2-aminoanthracene showed a negative correlation with activity in induced samples.
利用未处理大鼠和经多氯联苯混合物1254处理大鼠的肝脏S9，在平板掺入试验中研究了苯并[a]芘和2-氨基蒽对鼠伤寒沙门氏菌TA98的致突变性。随着时间的推移，对肝脏S9蛋白、芳烃羟化酶（AHH）和细胞色素P448/450的诱导情况进行了跟踪。诱导过程中蛋白质浓度没有变化；多氯联苯处理后第1、3、5和7天，AHH和细胞色素水平升高。经多氯联苯处理后，苯并[a]芘的致突变性增强，而2-氨基蒽的致突变性降低。平板上苯并[a]芘的致突变性与AHH和细胞色素水平呈正相关；2-氨基蒽与诱导样品中的活性呈负相关。
