Scheer Nico, Riedl Iris, Warren J T, Kuwada John Y, Campos-Ortega José A
Institut für Entwicklungsbiologie, Universität zu Köln, 50923 Köln, Germany.
Mech Dev. 2002 Mar;112(1-2):9-14. doi: 10.1016/s0925-4773(01)00621-9.
Using a temperature-inducible hsp70:Gal4 activator and UAS:myc-notch1a-intra as effector, we determined quantitatively the kinetics of expression of both transgenes and analysed the effects of varying their expressivity on several phenotypic traits in the developing zebrafish. hsp70:Gal4 is transcribed within 15 min after temperature-mediated induction, but Gal4 RNA decays rapidly. The Gal4 protein was found to be quite stable, as functional Gal4, which was detectable 1.5 h after heat shock (HS), persisted for at least 13 h. myc-notch1a-intra RNA is expressed approximately 1.5 h after HS, but unlike the Gal4 RNA, it was found to be very stable; it continues to accumulate during the succeeding 17 h after HS. Fully penetrant phenotypic effects are obtained after a relatively long activator induction with a 30-min HS.
我们使用温度诱导型hsp70:Gal4激活因子和UAS:myc-notch1a-intra作为效应物，定量测定了两个转基因的表达动力学，并分析了改变它们的表达量对斑马鱼发育过程中几个表型特征的影响。hsp70:Gal4在温度介导的诱导后15分钟内转录，但Gal4 RNA迅速降解。发现Gal4蛋白相当稳定，因为在热休克（HS）后1.5小时可检测到的功能性Gal4持续至少13小时。myc-notch1a-intra RNA在HS后约1.5小时表达，但与Gal4 RNA不同，它非常稳定；在HS后的后续17小时内它持续积累。通过30分钟的HS进行相对较长时间的激活因子诱导后，可获得完全显性的表型效应。
