Libelius R, Eaker D, Karlsson E
J Neural Transm. 1975;37(2):165-74. doi: 10.1007/BF01663631.
Preparations of 3H-monoacetylated derivatives of Naja naja siamensis neurotoxin siamensis 3 were found to be resistant to degradation and deacetylation during in vitro muscle incubation. A low rate of free 3H-acetate (less than 0.4%) was present in the preparations, but should not interfere with the binding of toxin to cholinergic receptors in mouse extensor digitorum longus muscles in vitro. The binding of toxin to cholinergic receptors in mouse extensor digitorum longus muscle was essentially irreversible. d-Tubocurarine antagonized the binding of toxin in both innervated and denervated muscles. Administration of actinomycin D one day after denervation prevented the appearance of new toxin binding sites in the muscles.
眼镜蛇泰国亚种神经毒素暹罗 3 的 3H - 单乙酰化衍生物制剂在体外肌肉孵育过程中被发现对降解和脱乙酰化具有抗性。制剂中游离 3H - 乙酸盐的含量较低（低于 0.4%），但这不应对毒素在体外与小鼠趾长伸肌中的胆碱能受体结合产生干扰。毒素与小鼠趾长伸肌中胆碱能受体的结合基本上是不可逆的。d - 筒箭毒碱可拮抗毒素在神经支配和去神经支配肌肉中的结合。去神经支配一天后给予放线菌素 D 可阻止肌肉中新毒素结合位点的出现。
