Paniukov A N, Smirnov V A
Biull Eksp Biol Med. 1980 May;89(5):574-5.
3H-monoacetyl derivative of the major neurotoxin of venom obtained from Naja naja oxiana was used as a marker of the nicotinic cholinoreceptor (N-ChR). It was shown that binding of neurotoxin with the membranes of bovine caudate nuclei unlike binding with the membranes of the rabbit gastrocnemius muscles is less strong and cannot be blocked completely with high concentrations of neurotoxin and d-tubocurarine. Serum of the rabbits immmunized with the protein preparation isolated from the membranes of caudate nuclei inhibited the binding of the marker by the membranes of caudate nuclei to a greater measure than by muscle membranes. It is concluded that the marker in question can be used for detecting N-ChR in caudate nuclei and that this cholinoreceptor differs from that of muscles.
从中亚眼镜蛇毒液中提取的主要神经毒素的3H-单乙酰衍生物被用作烟碱型胆碱受体（N-ChR）的标记物。结果表明，与兔腓肠肌膜结合不同，神经毒素与牛尾状核膜的结合较弱，且高浓度的神经毒素和d-筒箭毒碱不能完全阻断这种结合。用从尾状核膜分离的蛋白质制剂免疫的兔血清对尾状核膜标记物结合的抑制作用比对肌肉膜标记物结合的抑制作用更大。由此得出结论，所讨论的标记物可用于检测尾状核中的N-ChR，且这种胆碱受体与肌肉中的不同。
