Gonchar D A, Dedkov V S, Verkhozina V A, Kusner Iu S, Shevchenko A V, Degtiarev S Kh
SibEnzyme, Novosibirsk, Russia.
Prikl Biokhim Mikrobiol. 1998 Mar-Apr;34(2):139-41.
A new restriction endonuclease Sse9I was isolated from the bacterial strain Sporosarcina sp. 9D. The enzyme belongs to Type II restrictases and recognizes the tetranucleotide sequence 5'-AATT-3'. The enzyme cleaves DNA before the first adenine residue, so it is a true isoschizomer of Tsp509I restrictase. However, unlike the prototype, Sse9I digests DNA at 55 degrees C and loses its activity after 20 min storage at 65 degrees C.
