[链霉菌属48中II型限制性内切酶位点特异性的测定]
[The determination of the site specificity of type-II restriction endonuclease in Streptomyces sp. 48].
作者信息
Luk'ianchuk V V, Polishchuk L V, Stryzhkova H M, Kopieĭko O P, Matseliukh B P
机构信息
Institute of Microbiology and Virology, National Academy of Sciences of Ukraine, Kyiv.
出版信息
Mikrobiol Z. 1999 Jan-Feb;61(1):80-3.
The activity of restriction endonuclease which splits DNA of lambda and T7 phages into 8 fragments has been detected in the mycelium lysates of the 2-days culture of Streptomyces sp. 48. The fragments dimensions according to the data of agarose gel electrophoresis, coincide with those of AsuII--fragments of DNA of lambda and T7 phages. This allows the studied Ssp48 restrictase to be considered the isoschizomer of AsuII restriction endonuclease.
在链霉菌属48菌株两天培养物的菌丝体裂解物中，检测到一种能将λ噬菌体和T7噬菌体的DNA切割成8个片段的限制性内切酶活性。根据琼脂糖凝胶电泳数据，这些片段的大小与λ噬菌体和T7噬菌体DNA的AsuII片段的大小一致。这使得所研究的Ssp48限制性酶被认为是AsuII限制性内切酶的同裂酶。
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