牛乳头瘤病毒1型的E1蛋白对于维持病毒质粒DNA复制并非必需。

E1 protein of bovine papillomavirus 1 is not required for the maintenance of viral plasmid DNA replication.

作者信息

Kim Kitai, Lambert Paul F

机构信息

McArdle Laboratory for Cancer Research, University of Wisconsin Medical School, Madison, Wisconsin 53706, USA.

出版信息

Virology. 2002 Feb 1;293(1):10-4. doi: 10.1006/viro.2001.1305.

DOI:10.1006/viro.2001.1305

PMID:11853393

Abstract

Derivatives of bovine papillomavirus 1 (BPV1) with temperature-sensitive and dominant-negative mutation the E1 gene were used to determine the requirement for E1 in the maintenance of viral plasmid DNA replication. The abilities of these mutant BPV1 genomes to replicate as nuclear plasmids were monitored at permissive (32 degrees C) and nonpermissive (37 degrees C) temperatures in mouse C127 cells. We found that the temperature-sensitive E1 mutant BPV1 genomes replicate as nuclear plasmids as efficiently as does wild-type BPV1 in C127 cells after shifting to the nonpermissive temperature. These findings indicate that BPV1 does not require E1 for the maintenance of viral plasmids.

摘要

利用具有温度敏感性和显性负性突变的牛乳头瘤病毒1型（BPV1）E1基因衍生物，来确定E1在维持病毒质粒DNA复制中的必要性。在小鼠C127细胞中，于允许温度（32摄氏度）和非允许温度（37摄氏度）下监测这些突变BPV1基因组作为核质粒进行复制的能力。我们发现，温度敏感型E1突变BPV1基因组在转移至非允许温度后，在C127细胞中作为核质粒进行复制的效率与野生型BPV1一样高。这些发现表明，BPV1维持病毒质粒并不需要E1。