Guccione Ernesto, Massimi Paola, Bernat Agnieszka, Banks Lawrence
International Centre for Genetic Engineering and Biotechnology, Padriciano 99, I-34012 Trieste, Italy.
Virology. 2002 Feb 1;293(1):20-5. doi: 10.1006/viro.2001.1290.
We have compared the intracellular location of the HPV E6 and E7 proteins from high- and low-risk virus types. While high-risk HPV E7 displays diffuse nuclear expression, low-risk E7 has a nuclear punctuate pattern of expression. Similarly, while high-risk E6 is expressed throughout the cell, low-risk E6 is again predominantly nuclear with a punctuate pattern of expression. Both low-risk viral oncoproteins show colocalization with PML, whereas high-risk viral proteins do not. Finally, inhibition of the proteasome pathway results in a dramatic nuclear accumulation of high-risk E6 protein. These results demonstrate fundamental differences in the localization of these viral oncoproteins within the cell and offer alternative explanations for their respective differences in pathology.
我们比较了高危型和低危型病毒的人乳头瘤病毒E6和E7蛋白在细胞内的定位。高危型人乳头瘤病毒E7呈现弥漫性核表达，而低危型E7具有核点状表达模式。同样，高危型E6在整个细胞中表达，而低危型E6主要也是在细胞核中表达，呈点状模式。两种低危型病毒癌蛋白均与早幼粒细胞白血病蛋白（PML）共定位，而高危型病毒蛋白则不然。最后，蛋白酶体途径的抑制导致高危型E6蛋白在细胞核中大量积累。这些结果表明这些病毒癌蛋白在细胞内定位存在根本差异，并为它们在病理学上的各自差异提供了不同的解释。
