Karantonis Haralabos C, Antonopoulou Smaragdi, Demopoulos Constantinos A
Faculty of Chemistry, National and Kapodistrian University of Athens, Panepistimioupolis, 15771 Athens, Greece.
J Agric Food Chem. 2002 Feb 27;50(5):1150-60. doi: 10.1021/jf010923t.
Many epidemiological studies suggest that vegetable oils and especially olive oil present a protective effect against atherosclerosis. In this study, total lipids (TL) of Greek olive oils and seed oils of four kinds, namely, soybean, corn, sunflower, and sesame oil, were separated into total polar lipids (TPL) and total neutral lipids (TNL) via a novel extraction procedure. TPL and TNL of olive oil were fractionated by HPLC for further study. Each lipid fraction from HPLC separation along with TL, TPL, and TNL lipid samples from oils were tested in vitro for their capacity to induce or to inhibit washed rabbit platelet aggregation. Comparison between olive and seed oils supports the superiority of olive oil as high levels of platelet activating factor (PAF) antagonists have been detected, mainly in TPL. In addition, the structure of the most active fraction from olive oil was elucidated, as a glycerol-glycolipid. Because it has already been reported that PAF plays a pivotal role in atherogenesis, the existence of PAF agonists and antagonists in vegetable oils may explain their protective role against atherosclerosis.
许多流行病学研究表明，植物油尤其是橄榄油对动脉粥样硬化具有保护作用。在本研究中，通过一种新颖的提取方法，将希腊橄榄油以及大豆油、玉米油、葵花籽油和芝麻油这四种种子油的总脂质（TL）分离为总极性脂质（TPL）和总中性脂质（TNL）。橄榄油的TPL和TNL通过高效液相色谱法（HPLC）进行分离以作进一步研究。来自HPLC分离的每个脂质组分以及来自各种油的TL、TPL和TNL脂质样品在体外测试其诱导或抑制洗涤过的兔血小板聚集的能力。橄榄油和种子油之间的比较支持了橄榄油的优越性，因为已检测到高水平的血小板活化因子（PAF）拮抗剂，主要存在于TPL中。此外，已阐明来自橄榄油的最具活性的组分的结构为甘油糖脂。由于已有报道称PAF在动脉粥样硬化的发生中起关键作用，植物油中PAF激动剂和拮抗剂的存在可能解释了它们对动脉粥样硬化的保护作用。
