Burgeff Caroline, Liljegren Sarah J, Tapia-López Rosalinda, Yanofsky Martin F, Alvarez-Buylla Elena R
Instituto de Ecologia, UNAM, Mexico DF, Mexico.
Planta. 2002 Jan;214(3):365-72. doi: 10.1007/s004250100637.
Although MADS-box genes involved in flower and fruit development have been well characterized, the function of MADS-box genes expressed in vegetative structures has yet to be explored. At least seven members of this family are grouped in clades of genes that are preferentially expressed in roots of Arabidopsis thaliana (L.) Heynh.. We report here the cloning of the AGL21 MADS-box gene, which belongs to the ANR1 clade, and the mRNA in situ expression patterns of this and two other root MADS-box genes. AGL17 appears to be a lateral root cap marker in the root tip, and towards the elongation zone this gene is expressed in the epidermal cells. AGL21 is highly expressed in lateral root primordia and it has a punctate expression pattern in the primary root meristem. AGL12 also has a punctate expression pattern in the primary root meristem. AGL12 and AGL21 are also expressed in the central cylinder of differentiated roots and both are expressed in developing embryos. This study, combined with previous phylogenetic analyses, indicates that these MADS-box genes may play distinct regulatory roles during root development.
尽管参与花和果实发育的MADS-box基因已得到充分表征，但在营养器官中表达的MADS-box基因的功能尚未得到探索。该家族中至少有七个成员聚集在拟南芥根中优先表达的基因分支中。我们在此报告属于ANR1分支的AGL21 MADS-box基因的克隆，以及该基因和其他两个根MADS-box基因的mRNA原位表达模式。AGL17似乎是根尖侧根冠的标记，在伸长区，该基因在表皮细胞中表达。AGL21在侧根原基中高度表达，并且在初生根分生组织中具有点状表达模式。AGL12在初生根分生组织中也具有点状表达模式。AGL12和AGL21也在分化根的中柱中表达，并且在发育中的胚胎中均有表达。这项研究与先前的系统发育分析相结合，表明这些MADS-box基因可能在根发育过程中发挥不同的调控作用。
