Shao Zhengwei, He Minhong, Zeng Zhipeng, Chen Yanzhu, Hanna Amoanimaa-Dede, Zhu Hongbo
College of Coastal Agricultural Sciences, Guangdong Ocean University, Zhanjiang, China.
Front Genet. 2021 Nov 17;12:750137. doi: 10.3389/fgene.2021.750137. eCollection 2021.
MADS-box gene, one of the largest transcription factor families in plants, is a class of transcription factors widely present in eukaryotes. It plays an important role in plant growth and development and participates in the growth and development of flowers and fruits. Sweet potato is the seventh most important food crop in the world. Its tuberous roots, stems, and leaves contain a large number of proteins, lipids, carotenoids, anthocyanins, conjugated phenolic acids, and minerals, which have high edible, forage, and medicinal value, and is also an important energy crop. At present, MADS-box genes in sweet potato have rarely been reported, and there has been no study on the genome-wide identification and classification of MADS-box genes in . This study provided the first comprehensive analysis of sweet potato MADS-box genes. We identified 95 MADS-box genes, analyzed the structure and protein of sweet potato MADS-box genes, and categorized them based on phylogenetic analysis with MADS-box proteins. Chromosomal localization indicated an unequal number of MADS-box genes in all 14 chromosomes except LG3, with more than 10 MADS-box genes located on chromosomes LG7, LG11, and LG15. The MADS domain and core motifs of the sweet potato MADS-box genes were identified by motif analysis. We identified 19 MADS-box genes with collinear relationships and analyzed duplication events. -acting elements, such as light-responsive, auxin-responsive, drought-inducible, and MeJA-responsive elements, were found in the promoter region of the MADS-box genes in sweet potato, which further indicates the basis of MADS-box gene regulation in response to environmental changes and hormones. RNA-seq suggested that sweet potato MADS-box genes exhibit tissue-specific expression patterns, with 34 genes highly expressed in sweet potato flowers and fruits, and 19 genes highly expressed in the tuberous root, pencil root, or fibrous root. qRT-PCR again validated the expression levels of the 10 genes and found that , , , , and were highly expressed in the tuberous root or fibrous root, and , , and were highly expressed in the fruit. In this study, the molecular basis of MADS-box genes of sweet potato was analyzed from various angles. The effects of MADS-box genes on the growth and development of sweet potato were investigated, which may provide a certain theoretical basis for molecular breeding of sweet potato.
MADS盒基因是植物中最大的转录因子家族之一，是一类广泛存在于真核生物中的转录因子。它在植物生长发育中起重要作用，参与花和果实的生长发育。甘薯是世界上第七大重要粮食作物。其块根、茎和叶含有大量蛋白质、脂质、类胡萝卜素、花青素、共轭酚酸和矿物质，具有很高的食用、饲料和药用价值，也是一种重要的能源作物。目前，甘薯中MADS盒基因鲜有报道，尚未有对甘薯中MADS盒基因进行全基因组鉴定和分类的研究。本研究首次对甘薯MADS盒基因进行了全面分析。我们鉴定出95个MADS盒基因，分析了甘薯MADS盒基因的结构和蛋白质，并基于与其他MADS盒蛋白的系统发育分析对它们进行了分类。染色体定位表明，除LG3外，所有14条染色体上的MADS盒基因数量不等，LG7、LG11和LG15染色体上有超过10个MADS盒基因。通过基序分析鉴定了甘薯MADS盒基因的MADS结构域和核心基序。我们鉴定出19个具有共线关系的MADS盒基因并分析了重复事件。在甘薯MADS盒基因的启动子区域发现了光响应、生长素响应、干旱诱导和茉莉酸甲酯响应等顺式作用元件，这进一步表明了MADS盒基因响应环境变化和激素调控的基础。RNA测序表明，甘薯MADS盒基因表现出组织特异性表达模式，34个基因在甘薯花和果实中高表达，19个基因在块根、细根或须根中高表达。qRT-PCR再次验证了10个基因的表达水平，发现 、 、 、 和 在块根或须根中高表达， 、 和 在果实中高表达。本研究从多个角度分析了甘薯MADS盒基因的分子基础。研究了MADS盒基因对甘薯生长发育的影响，这可能为甘薯分子育种提供一定的理论依据。
