Beyer W F, Gleason D D
J Pharm Sci. 1975 Sep;64(9):1557-60. doi: 10.1002/jps.2600640932.
An automated system for high-pressure liquid chromatography was developed. The system is built around commercial modules wherever possible, modified to varying degrees. An automatic sampler, a sample pump, a high-pressure sampling valve, a recorder with an integrator, and a high-pressure liquid chromatograph comprise the commercial instruments. Relays, solenoid valves, and timers control chromatographic events, i.e., duration of sampling and rinse, mobile phase pump refill, sample injection, and chromatographic time. The automated system is dependable over long periods of unattended operation. With the 40-sample capacity of the sample tray and the last sample stop capability, the automated system produces, for example, 40 20-min chromatograms in approximately 13 hr of unattended operation. Data demonstrate the reliability and utility of the system.
开发了一种用于高压液相色谱的自动化系统。该系统尽可能围绕商业模块构建，并在不同程度上进行了修改。自动进样器、样品泵、高压进样阀、带积分仪的记录仪和高压液相色谱仪构成了商业仪器。继电器、电磁阀和定时器控制色谱事件，即进样和冲洗时间、流动相泵再填充、样品注入和色谱时间。该自动化系统在长时间无人值守操作下可靠运行。凭借样品盘40个样品的容量和最后一个样品停止功能，该自动化系统在大约13小时的无人值守操作中可产生40个20分钟的色谱图。数据证明了该系统的可靠性和实用性。
