Okada Kyoji, Itoi Eiji, Miyakoshi Naohisa, Nakajima Mizuho, Suzuki Toshio, Nishida Jun
Department of Orthopedic Surgery, Akita University School of Medicine, Akita 010-8543, Japan.
Jpn J Cancer Res. 2002 Feb;93(2):216-22. doi: 10.1111/j.1349-7006.2002.tb01261.x.
The antitumor effects of piroxicam, a non-steroidal anti-inflammatory drug, on sarcoma 180 cells under ultrasonic irradiation were examined in a mouse air pouch model. When piroxicam was added to sarcoma 180 suspension under ultrasound irradiation (2 MHz, 10 W, 120 s), the mortality rate of tumor cells immediately after the irradiation and the survival rate of mice were significantly higher than those when ultrasound alone was applied, and these effects of piroxicam were dose-dependent. When D-mannitol was used with piroxicam, the mortality rate of the tumors cells after the irradiation was comparable with that when piroxicam alone was applied, but when L-histidine was used concurrently, the antitumor effect was significantly lower than that when piroxicam alone was applied. Histological examinations one week after the ultrasound irradiation in the presence of piroxicam showed sparse tumor tissue in the air pouch and normal appearance of the air pouch and surrounding tissue. The findings suggest that piroxicam enhances the anti-tumor effects of ultrasound in vivo by increasing the production of singlet oxygen without damage to tissue surrounding the tumor.
在小鼠气袋模型中，研究了非甾体抗炎药吡罗昔康在超声照射下对肉瘤180细胞的抗肿瘤作用。当在超声照射（2MHz，10W，120秒）下将吡罗昔康添加到肉瘤180悬浮液中时，照射后肿瘤细胞的死亡率和小鼠的存活率显著高于仅应用超声时，且吡罗昔康的这些作用呈剂量依赖性。当D-甘露醇与吡罗昔康一起使用时，照射后肿瘤细胞的死亡率与仅应用吡罗昔康时相当，但当同时使用L-组氨酸时，抗肿瘤作用明显低于仅应用吡罗昔康时。在有吡罗昔康存在的情况下进行超声照射一周后的组织学检查显示，气袋内肿瘤组织稀疏，气袋及周围组织外观正常。这些发现表明，吡罗昔康通过增加单线态氧的产生来增强超声在体内的抗肿瘤作用，而不会对肿瘤周围组织造成损伤。
