Luo Y, Long J M, Spangler E L, Longo D L, Ingram D K, Weng N P
Laboratory of Immunology, National Institute on Aging, National Institutes of Health, Baltimore, MD 21224, USA.
J Mol Neurosci. 2001 Dec;17(3):397-404. doi: 10.1385/JMN:17:3:397.
Long-term memory formation requires de novo RNA and protein synthesis. To assess gene-expression changes associated with learning and memory processes, we used cDNA microarray to analyze hippocampal gene expression in male Fischer-344 rats following training in a multiunit T-maze. Here, we report the identification of 28 clones (18 known genes and 10 ESTs) for which expression increased after the maze learning. Some of the known genes appear to be involved in Ca2+ signaling, Ras activation, kinase cascades, and extracellular matrix (ECM) function, which may regulate neural transmission, synaptic plasticity, and neurogenesis. The gene-expression profile presented here provides the groundwork for future, more focused research to elucidate the contribution of these genes in learning and memory processes.
长期记忆的形成需要从头合成RNA和蛋白质。为了评估与学习和记忆过程相关的基因表达变化，我们使用cDNA微阵列分析了雄性Fischer-344大鼠在多单元T迷宫训练后的海马基因表达。在此，我们报告了28个克隆（18个已知基因和10个EST）的鉴定结果，这些克隆在迷宫学习后表达增加。一些已知基因似乎参与Ca2+信号传导、Ras激活、激酶级联反应和细胞外基质（ECM）功能，这可能调节神经传递、突触可塑性和神经发生。这里呈现的基因表达谱为未来更有针对性的研究奠定了基础，以阐明这些基因在学习和记忆过程中的作用。
