Pomponio Romeo, Gotti Roberto, Hudaib Mohammad, Cavrini Vanni
Dipartimento di Scienze Farmaceutiche, Università di Bologna, Italy.
J Chromatogr A. 2002 Feb 1;945(1-2):239-47. doi: 10.1016/s0021-9673(01)01488-1.
A micellar electrokinetic chromatographic (MEKC) method was developed for the separation of ten phenolic acids including cichoric acid and caftaric acids, specific marker phytochemicals of Echinacea purpurea. The MEKC method involved the use of 70 mM sodium deoxycholate (SDC) in 40 mM borate (pH 9.2) buffer and UV detection at 300 nm. The bile acid was used as biosurfactant able to provided a micellar system with different and more selective properties than sodium dodecyl sulfate. The effects of SDC and borate concentration and buffer pH on the analyte resolution were evaluated. The validated method was applied to the determination of cichoric acid and related compounds in E. purpurea root extracts, and in commercial E. purpurea based dried extracts and tablets.
建立了一种胶束电动色谱（MEKC）方法，用于分离包括紫锥菊苷和咖啡酸在内的10种酚酸，这些是紫锥菊的特定标志性植物化学物质。该MEKC方法使用在40 mM硼酸盐（pH 9.2）缓冲液中的70 mM脱氧胆酸钠（SDC），并在300 nm处进行紫外检测。胆汁酸用作生物表面活性剂，能够提供具有与十二烷基硫酸钠不同且更具选择性的性质的胶束体系。评估了SDC和硼酸盐浓度以及缓冲液pH对分析物分离度的影响。该验证方法用于测定紫锥菊根提取物、市售紫锥菊干提取物和片剂中的紫锥菊苷及相关化合物。
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