Department of Medicinal Chemistry and Natural Medicine Chemistry, College of Pharmacy, Harbin Medical University, No. 157 Baojian Road, Nangang District, Harbin 150081, China.
Key Laboratory of Chinese Materia Medica (Ministry of Education), Heilongjiang University of Chinese Medicine, Harbin 150040, China.
Molecules. 2017 Sep 7;22(9):1494. doi: 10.3390/molecules22091494.
A rapid and sensitive Ultra High Performance Liquid Chromatography Electrospray Ionization Tandem Mass Spectrometry (UHPLC-ESI-MS/MS) method was developed and validated to simultaneously determine the concentration of seven phenolic acids (syringic acid, ferulic acid, caffeic acid, vanillic acid, -coumaric acid, 3,4-dihydroxybenzoic acid and 4-hydroxybenzoic acid) in rat plasma after oral administration of extract. After mixing with the internal standard (IS), butylparaben, plasma samples were prepared by liquid-liquid extraction with ethyl acetate. The separation was performed using the Agilent Eclipse Plus C column (1.8 μm, 2.1 mm × 50 mm) with a gradient system consisting of solution A (0.1% acetic acid in water) and solution B (methanol) at a flow rate of 0.3 mL/min. The detection was accomplished by a multiple reaction monitoring (MRM) mode with electrospray ionization (ESI). The method was validated in terms of linearity, precision, accuracy, extraction recovery, matrix effect and stability. This method was successfully applied to study the pharmacokinetic properties of the seven compounds after oral administration of extract in rats.
建立并验证了一种快速灵敏的超高效液相色谱-电喷雾串联质谱（UHPLC-ESI-MS/MS）法，用于同时测定大鼠灌胃 提取物后血浆中 7 种酚酸（丁香酸、阿魏酸、咖啡酸、香草酸、-香豆酸、3,4-二羟基苯甲酸和 4-羟基苯甲酸）的浓度。用内标（对羟基苯甲酸丁酯）处理混合血浆样品后，采用乙酸乙酯进行液液萃取。采用 Agilent Eclipse Plus C 柱（1.8 μm，2.1 mm×50 mm），以 0.1%乙酸水溶液（A 液）和甲醇（B 液）为流动相，在 0.3 mL/min 的流速下进行梯度洗脱。采用电喷雾（ESI）多反应监测（MRM）模式进行检测。该方法在线性、精密度、准确度、提取回收率、基质效应和稳定性方面均得到了验证。该方法成功应用于研究大鼠灌胃 提取物后 7 种化合物的药代动力学性质。
