Middleton Andrew M., Chadwick Maureen V., Gaya Harold
Department of Microbiology, Royal Brompton Hospital, London, UK.
Clin Microbiol Infect. 1997 Feb;3(6):668-671. doi: 10.1111/j.1469-0691.1997.tb00476.x.
To investigate the ability of a commercial acridinium ester-labeled DNA probe (AccuProbe, Gen-Probe Inc., USA) to detect the presence of Mycobacterium tuberculosis complex in a mixed culture with Mycobacterium avium complex. METHODS: The density of organisms required to produce a positive result for Mycobacterium tuberculosis complex alone in broth culture was compared with the density required to produce a positive result in the presence of Mycobacterium avium complex. RESULTS: A threshold density of 1.5 x 106 CFU/mL was required for detection of Mycobacterium tuberculosis and this threshold remained unaltered in the presence of Mycobacterium avium complex. The presence of Mycobacterium avium complex had no effect on detection of Mycobacterium tuberculosis in a mixed broth culture incubated and probed over a 21-day period. CONCLUSIONS: The findings of the study suggest that the presence of Mycobacterium avium complex has no effect on the detection of Mycobacterium tuberculosis and that the Accuprobe test is potentially capable of detecting a dual infection with organisms of both complexes.
研究一种市售的吖啶酯标记DNA探针（AccuProbe，美国Gen-Probe公司）在与鸟分枝杆菌复合群混合培养物中检测结核分枝杆菌复合群的能力。方法：比较在肉汤培养中单独使结核分枝杆菌复合群产生阳性结果所需的菌密度与在有鸟分枝杆菌复合群存在时产生阳性结果所需的菌密度。结果：检测结核分枝杆菌需要的阈值密度为1.5×106 CFU/mL，在有鸟分枝杆菌复合群存在时该阈值保持不变。在21天的培养和检测期间，鸟分枝杆菌复合群的存在对混合肉汤培养中结核分枝杆菌的检测没有影响。结论：该研究结果表明，鸟分枝杆菌复合群的存在对结核分枝杆菌的检测没有影响，且Accuprobe试验有可能检测出两种复合群菌株的双重感染。
