Wen Xiao Bo, Chen Li Qiao, Zhou Zhong Liang, Ai Chun Xiang, Deng Glenn Y
Department of Biology, East China Normal University, Shanghai 200062, PR China.
Comp Biochem Physiol A Mol Integr Physiol. 2002 Mar;131(3):675-81. doi: 10.1016/s1095-6433(01)00515-3.
The effect of feeding three semi-purified diets containing different lipid sources (anchovy oil, soybean oil and pork lard) on fecundity, hatchability and egg fatty acid composition of Chinese mitten-handed crab (Eriocheir sinensis) broodstock was compared with a fresh clam diet in a 6-month feeding trial. Broodstock crabs fed the diet containing pork lard showed poor fecundity and low hatchability. Crabs fed the diet containing soybean oil showed improved fecundity; however, no significant improvement in hatchability was observed. Broodstock fed the diet containing anchovy oil showed the highest fecundity and egg hatchability. Eggs from broodstock fed anchovy oil as sole dietary lipid had a higher n-3 polyunsaturated fatty acid (PUFA) content (33.3%) compared with those of crabs fed diets with soybean oil (20.1%) and pork lard (16.3%) as lipid sources. The results indicate a close correlation between: (1) the 20:5n-3 content of the egg lipid and fecundity; (2) the 22:6n-3 content and hatchability; and (3) fecundity, hatchability and n-3/n-6 fatty acid ratio. The results also suggest that each of these n-3 HUFAs may play different and specific roles in crab reproduction and that either must be adequate in the broodstock diet.
在一项为期6个月的投喂试验中，将含有不同脂质来源（鳀鱼油、大豆油和猪油）的三种半纯化饲料投喂中华绒螯蟹亲体，与投喂新鲜蛤类饲料进行比较，研究其对中华绒螯蟹亲体繁殖力、孵化率和卵脂肪酸组成的影响。投喂含猪油饲料的亲体蟹繁殖力差且孵化率低。投喂含大豆油饲料的蟹繁殖力有所提高；然而，未观察到孵化率有显著改善。投喂含鳀鱼油饲料的亲体繁殖力和卵孵化率最高。以鳀鱼油作为唯一膳食脂质来源投喂的亲体所产的卵，其n-3多不饱和脂肪酸（PUFA）含量（33.3%）高于以大豆油（20.1%）和猪油（16.3%）作为脂质来源投喂的蟹所产的卵。结果表明：（1）卵脂质中20:5n-3的含量与繁殖力之间存在密切相关性；（2）22:6n-3的含量与孵化率之间存在密切相关性；（3）繁殖力、孵化率与n-
