Rosch Raphael, Klinge Uwe, Si Zhongyi, Junge Karsten, Klosterhalfen Bernd, Schumpelick Volker
BMC Med Genet. 2002;3:2. doi: 10.1186/1471-2350-3-2. Epub 2002 Feb 19.
Abnormal collagen metabolism is thought to play an important role in the development of primary inguinal hernia. This is underlined by detection of altered collagen metabolism and structural changes of the tissue in patients with primary inguinal hernia. However, it is still unknown whether these alterations reflect a basic dysfunction of the collagen synthesis, or of collagen degradation.
In the present study, we analysed type I and type III procollagen messenger ribonucleic acid (mRNA) and MMP-1 and MMP-13 mRNA in cultured fibroblasts from the skin of patients with primary inguinal hernia, and from patients without hernia (controls) by reverse transcription polymerase chain reaction (RT-PCR) and Northern Blot.
The results indicated that the ratio of type I to type III procollagen mRNA was decreased in patients with primary hernia, showing significant differences as compared to controls (p = 0.01). This decrease was mainly due to the increase of type III procollagen mRNA. Furthermore, RT-PCR analysis revealed that the expression of MMP-1 mRNA in patients with primary hernia is equivalent to that of controls (p > 0.05). In addition, MMP-13 mRNA is expressed neither in patients with primary hernia nor in controls.
We concluded that abnormal change of type I and type III collagen mRNAs contribute to the development of primary inguinal hernia, whereas the expressions of MMP-1 and MMP-13 mRNA appears not to be involved in the development of primary inguinal hernia. Thus, the knowledge on the transcriptional regulation of collagen in patients with primary inguinal hernia may help to understand the pathogenesis of primary inguinal hernia, and implies new therapeutic strategies for this disease.
异常的胶原蛋白代谢被认为在原发性腹股沟疝的发生发展中起重要作用。原发性腹股沟疝患者组织中胶原蛋白代谢改变及结构变化的检测证实了这一点。然而，这些改变是反映胶原蛋白合成的基本功能障碍还是胶原蛋白降解的基本功能障碍仍不清楚。
在本研究中，我们通过逆转录聚合酶链反应（RT-PCR）和Northern印迹分析了原发性腹股沟疝患者及无疝患者（对照组）皮肤培养成纤维细胞中I型和III型前胶原信使核糖核酸（mRNA）以及基质金属蛋白酶-1（MMP-1）和基质金属蛋白酶-13（MMP-13）mRNA的情况。
结果表明，原发性疝患者I型与III型前胶原mRNA的比值降低，与对照组相比有显著差异（p = 0.01）。这种降低主要是由于III型前胶原mRNA的增加。此外，RT-PCR分析显示原发性疝患者中MMP-1 mRNA的表达与对照组相当（p > 0.05）。另外，原发性疝患者和对照组中均未检测到MMP-13 mRNA的表达。
我们得出结论，I型和III型胶原mRNA的异常变化促成了原发性腹股沟疝的发生，而MMP-1和MMP-13 mRNA的表达似乎与原发性腹股沟疝的发生无关。因此，了解原发性腹股沟疝患者胶原蛋白转录调控的知识可能有助于理解原发性腹股沟疝的发病机制，并为该疾病暗示新的治疗策略。
