通过戊二醛偶联和硼氢化钠还原将腺嘌呤核苷酸固定在多糖上。
The immobilization of adenine nucleotides on polysaccharides by using glutaraldehyde coupling and borohydride reduction.
作者信息
Gacesa P, Whish W J
出版信息
Biochem J. 1978 Oct 1;175(1):349-52. doi: 10.1042/bj1750349.
Abstract
Adenine nucleotides were immobilized on modified Sepharose 4B or Dextran T40 with glutaraldehyde and reduced with KBH4. Binding was dependent on pH and the nature of the amino group on the modified polysaccharide. ATP bound to soluble dextran retained coenzyme activity with glycerol kinase. Binding is proposed to occur via a Schiff base.
摘要
腺嘌呤核苷酸通过戊二醛固定在修饰的琼脂糖4B或葡聚糖T40上，并用硼氢化钾还原。结合取决于pH值和修饰多糖上氨基的性质。与可溶性葡聚糖结合的ATP对甘油激酶保留辅酶活性。推测结合是通过席夫碱发生的。
相似文献
1
The immobilization of adenine nucleotides on polysaccharides by using glutaraldehyde coupling and borohydride reduction.通过戊二醛偶联和硼氢化钠还原将腺嘌呤核苷酸固定在多糖上。
Biochem J. 1978 Oct 1;175(1):349-52. doi: 10.1042/bj1750349.
2
The preparation of stable enzyme-coenzyme complexes with endogenous catalytic activity.具有内源性催化活性的稳定酶-辅酶复合物的制备。
Biochem J. 1979 Jan 1;177(1):369-72. doi: 10.1042/bj1770369.
3
An essential role for constitutive Schiff base-forming ligands in antigen presentation to murine T cell clones.
J Immunol. 1990 Apr 15;144(8):2883-90.
4
The interaction of beta-cyclodextrin with dinucleoside phosphates.β-环糊精与二核苷磷酸的相互作用。
Biopolymers. 1974 May;13(5):909-17. doi: 10.1002/bip.1974.360130507.
5
Optimization of penicillin G acylase multipoint immobilization on to glutaraldehyde-chitosan beads.青霉素G酰化酶在戊二醛-壳聚糖珠上多点固定化的优化。
Biotechnol Appl Biochem. 2005 Jun;41(Pt 3):201-7. doi: 10.1042/BA20040061.
6
Haemoglobin solutions: reversibly bound oxygen and its effect upon the hypoxic heart.
Physiol Bohemoslov. 1986;35(3):193-8.
7
A new method for the study of glutaraldehyde-induced crosslinking properties in proteins with special reference to the reaction with amino groups.一种研究戊二醛诱导蛋白质交联特性的新方法，特别涉及与氨基的反应。
J Histochem Cytochem. 1978 May;26(5):412-4. doi: 10.1177/26.5.96177.
8
Studies on the nature of the regulation by purine nucleotides of adenine phosphoribosyltransferase and of hypoxanthine phosphoribosyltransferase from Ehrlich ascites-tumour cells.关于嘌呤核苷酸对艾氏腹水瘤细胞中腺嘌呤磷酸核糖转移酶和次黄嘌呤磷酸核糖转移酶调节性质的研究。
Biochem J. 1967 Apr;103(1):271-9. doi: 10.1042/bj1030271.
9
Methods of immobilization of proteins to polymethylmethacrylate.
Prep Biochem. 1985;15(5):309-19. doi: 10.1080/00327488508062448.
10
V. Efforts at stabilization of rat liver phenylalanine hydroxylase.
Res Commun Chem Pathol Pharmacol. 1979 Jul;25(1):153-64.
引用本文的文献
1
In vitro nuclear interactome of the HIV-1 Tat protein.HIV-1反式激活蛋白的体外核相互作用组
Retrovirology. 2009 May 19;6:47. doi: 10.1186/1742-4690-6-47.
2
Selective glutaraldehyde-mediated coupling of proteins to the 3'-adenine terminus of polymerase chain reaction products.蛋白质与聚合酶链反应产物3'-腺嘌呤末端的选择性戊二醛介导偶联。
J Biomol Tech. 2008 Jul;19(3):177-83.
3
The preparation of stable enzyme-coenzyme complexes with endogenous catalytic activity.具有内源性催化活性的稳定酶-辅酶复合物的制备。
Biochem J. 1979 Jan 1;177(1):369-72. doi: 10.1042/bj1770369.
本文引用的文献
1
The mechanism of action of aldolases. III. Schiff base formation with lysine.醛缩酶的作用机制。III. 与赖氨酸形成席夫碱
Biochem Biophys Res Commun. 1962 Sep 25;9:38-43. doi: 10.1016/0006-291x(62)90083-9.
2
Phosphorus assay in column chromatography.柱色谱法中的磷测定
J Biol Chem. 1959 Mar;234(3):466-8.
3
The direct linear plot. A new graphical procedure for estimating enzyme kinetic parameters.直接线性作图法。一种用于估算酶动力学参数的新的图形方法。
Biochem J. 1974 Jun;139(3):715-20. doi: 10.1042/bj1390715.
4
Affinity chromatography on immobilised adenosine 5'-monophosphate. 1. A new synthesis and some properties of an N6-immobilised 5'-AMP.固定化5'-单磷酸腺苷的亲和色谱法。1. N6固定化5'-AMP的一种新合成方法及某些性质
Eur J Biochem. 1974 Jan 16;41(2):329-33. doi: 10.1111/j.1432-1033.1974.tb03273.x.
5
A new immobilized NAD+ analogue, its application in affinity chromatography and as a functioning coenzyme.一种新型固定化NAD⁺类似物，其在亲和色谱中的应用以及作为一种起作用的辅酶的应用。
Eur J Biochem. 1973 Dec 3;40(1):187-93. doi: 10.1111/j.1432-1033.1973.tb03184.x.
6
The purification of nicotinamide nucleotide-dependent dehydrogenases on immobilized cofactors.基于固定化辅因子的烟酰胺核苷酸依赖性脱氢酶的纯化
Biochem J. 1973 Jul;133(3):507-13. doi: 10.1042/bj1330507.
7
A facile method for the preparation of N6-substituted ATP-Sepharose.一种制备N6-取代的ATP-琼脂糖凝胶的简便方法。
Nucleic Acids Res. 1975 Oct;2(10):1771-5. doi: 10.1093/nar/2.10.1771.
8
Immobilization of enzymes to agar, agarose, and Sephadex supports.酶固定于琼脂、琼脂糖和葡聚糖凝胶载体上。
Methods Enzymol. 1976;44:19-45. doi: 10.1016/s0076-6879(76)44005-3.
9
A novel method of coenzyme immobilization [proceedings].
Biochem Soc Trans. 1977;5(5):1407-8. doi: 10.1042/bst0051407.
10
Covalent immobilization of soluble and enzymically active adenine nucleotide coenzymes by a single step procedure.
J Biol Chem. 1977 Oct 10;252(19):6631-9.
Zotero 插件