Intorre L, Castells G, Cristòfol C, Bertini S, Soldani G, Arboix M
Department of Veterinary Clinics, Section of Pharmacology and Toxicology, University of Pisa, Pisa, Italy.
J Vet Pharmacol Ther. 2002 Feb;25(1):59-63. doi: 10.1046/j.1365-2885.2002.00374.x.
The residue depletion of thiamphenicol (TAP) was investigated in the sea-bass (Dicentrarchus labrax) after 5 days' treatment with medicated food at a dose of 15 or 30 mg/kg bw/day. Fish were sampled for blood and muscle + skin from 3 h until 14 days after treatment. Thiamphenicol concentrations were assayed by high performance liquid chromatography. Thiamphenicol concentrations measured 3 h after stopping treatment were 0.77 microg/mL and 0.91 (15 mg/kg dose) or 1.32 microg/mL and 1.47 microg/g (30 mg/kg dose), in plasma and muscle + skin, respectively. After a withdrawal of 3 days, plasma and tissue concentrations were: 0.08 microg/mL and 0.03 microg/g (lower dose) or 0.12 microg/mL and 0.06 microg/g (higher dose), respectively. Thiamphenicol was not detectable either in plasma or in tissues on days 7, 10 and 14 following withdrawal of the medicated food. Based on maximum residue levels (MRL) for TAP in fin fish, established at 50 microg/kg for muscle and skin in natural proportions, a withdrawal period of 5 and 6 days is proposed, after treatment at 15 or 30 mg/kg of TAP with medicated feed pellets, respectively, to avoid the presence of violative residues in the edible tissues of the sea-bass.
在用剂量为15或30毫克/千克体重/天的药饵处理鲈鱼（欧洲鲈鱼）5天后，研究了甲砜霉素（TAP）的残留消除情况。在处理后3小时至14天对鱼进行采血，并采集肌肉+皮肤样本。采用高效液相色谱法测定甲砜霉素浓度。停药3小时后，血浆和肌肉+皮肤中甲砜霉素浓度分别为0.77微克/毫升和0.91微克/克（15毫克/千克剂量组），或1.32微克/毫升和1.47微克/克（30毫克/千克剂量组）。停药3天后，血浆和组织中的浓度分别为：0.08微克/毫升和0.03微克/克（低剂量组），或0.12微克/毫升和0.06微克/克（高剂量组）。在停止投喂药饵后的第7、10和14天，血浆和组织中均未检测到甲砜霉素。根据为有鳍鱼类制定的甲砜霉素最大残留限量（MRL），即肌肉和皮肤按自然比例计算为50微克/千克，在用含15或30毫克/千克甲砜霉素的药饵颗粒处理后，分别建议停药期为5天和6天，以避免鲈鱼可食用组织中出现违规残留。
