Department of Pathology, Diagnostic and Veterinary Clinic, Faculty of Veterinary Medicine, University of Perugia, Perugia, Italy.
J Fish Dis. 2013 Aug;36(8):685-93. doi: 10.1111/j.1365-2761.2012.01437.x. Epub 2013 Feb 6.
An increase in fish production has consequently brought an increase in infectious diseases in fish farms. The use of chemotherapic drugs is the most effective instrument against common bacterial agents. The number of registered drugs for use in aquaculture is limited and often veterinary practitioners resort to the off-label use of chemotherapic agents authorized for different food-producing animal species. Florfenicol is well known for its outstanding effect against various pathogenic bacteria affecting fish, and therefore, it may be a useful drug for off-label use in aquaculture. The aim of this study was to evaluate the depletion of florfenicol and its major metabolite, florfenicol amine, from the edible tissue of two fish species, rainbow trout and sea bream, following treatment with medicated feed at a dosage of 10 mg kg(-1) of bw day(-1) , for 10 consecutive days. At prefixed time points after the end of administration (0.25, 1, 2, 3, 4, 6, 7, 10, 14 and 21 days after treatment), edible tissues (muscle plus adherent skin) from 15 individuals in each group were collected and analysed by HPLC, to determine concentration of the drug in the tissue. On the basis of the obtained concentrations, withdrawal times of florfenicol in the two species were calculated. The results indicate that a drug withdrawal time of 500 °C-day, as established by Directive 2004/28/EC, for off-label drug use is more than satisfactory to guarantee the healthiness of fish products against the risk of drug residues.
鱼类产量的增加导致了鱼类养殖场传染性疾病的增加。化疗药物的使用是对抗常见细菌的最有效手段。可用于水产养殖的注册药物数量有限，兽医通常会选择超说明书使用已批准用于不同食用动物物种的化疗药物。氟苯尼考因其对影响鱼类的各种病原菌的显著效果而广为人知，因此，它可能是水产养殖中超说明书使用的一种有用药物。本研究的目的是评估在以 10mg/kg bw/day 的剂量经饲用药物治疗 10 天后，两种鱼类（虹鳟和海鲈）的可食用组织中氟苯尼考及其主要代谢物氟苯尼考胺的耗尽情况。在给药结束后的预定时间点（治疗后 0.25、1、2、3、4、6、7、10、14 和 21 天），从每组 15 个个体中采集可食用组织（肌肉加附着皮肤），并通过 HPLC 进行分析，以确定组织中药物的浓度。根据获得的浓度，计算了两种鱼类中氟苯尼考的停药时间。结果表明，根据指令 2004/28/EC 规定的 500°C 天的停药时间，足以满足保证鱼类产品健康、避免药物残留风险的要求。
