Pfaar Harald, von Holst Alexander, Vogt Weisenhorn Daniela M, Brodski Claude, Guimera Jordi, Wurst Wolfgang
GSF-Research Center for Environment and Health, Institute for Mammalian Genetics, Ingolstädter Landstrasse 1, 85764 Neuherberg, Germany.
Dev Genes Evol. 2002 Feb;212(1):43-6. doi: 10.1007/s00427-001-0208-x. Epub 2002 Jan 31.
Here we describe the expression pattern of a previously unknown mouse gene mPet-1. The isolated cDNA codes for an ETS-domain transcription factor of 237 amino acids in length, which is localized to the nucleus. mPet-1 is a member of the winged helix transcription factor gene family like its rat homologue Pet-1 and the human homologue FEV. The start ATG of mPet-1 and the size of the predicted protein are identical to the human FEV. The mPet-1 protein is clearly smaller since it lacks the first 103 N-terminal amino acids of rat Pet-1. mPet- 1 is expressed in central serotonergic (5-hydroxytryptaminergic) neurons located in the mes-/metencephalic raphe nuclei from E11 on until adulthood. In these regions mPet-1 expression co-localizes precisely with the serotonin transporter (Sert),which it initially precedes. Interestingly, mPet-1 was not found in neurons transiently expressing Sert.
在此，我们描述了一个此前未知的小鼠基因mPet-1的表达模式。分离得到的cDNA编码一个长度为237个氨基酸的ETS结构域转录因子，该转录因子定位于细胞核。mPet-1与其大鼠同源物Pet-1和人类同源物FEV一样，是翼状螺旋转录因子基因家族的成员。mPet-1的起始ATG和预测蛋白质的大小与人类FEV相同。mPet-1蛋白明显较小，因为它缺少大鼠Pet-1的前103个N端氨基酸。从胚胎第11天直至成年，mPet-1在位于中脑/后脑缝际核的中枢5-羟色胺能神经元中表达。在这些区域，mPet-1的表达与5-羟色胺转运体（Sert）精确共定位，且mPet-1的表达最初先于Sert出现。有趣的是，在短暂表达Sert的神经元中未发现mPet-1。
