Brickwood Sarah J, Myers Fiona A, Chandler Simon P
Sangamo Biosciences Inc., Point Richmond Tech Center, Richmond, CA 04804, USA.
Mol Biotechnol. 2002 Jan;20(1):1-15. doi: 10.1385/MB:20:1:001.
The analysis of protein interactions with chromatin is vital for the understanding of DNA sequence recognition in vivo. Chromatin binding requires the interaction of proteins with DNA lying on the macromolecular protein surface of nucleosomes, a situation that can alter factor binding characteristics substantially when compared with naked DNA. It is therefore important to study these protein-DNA interactions in the context of a chromatin substrate, the more physiologically relevant binding situation. In this article we review techniques used in the investigation of protein interactions with defined nucleosomal templates.
对蛋白质与染色质相互作用的分析对于理解体内DNA序列识别至关重要。染色质结合需要蛋白质与位于核小体大分子蛋白质表面的DNA相互作用，与裸露的DNA相比，这种情况会极大地改变因子结合特性。因此，在染色质底物的背景下研究这些蛋白质 - DNA相互作用非常重要，这是更具生理相关性的结合情况。在本文中，我们综述了用于研究蛋白质与特定核小体模板相互作用的技术。
