Wu W T, Hsu Y L, Ko Y F, Yao L L
Department of Chemical Engineering, National Tsing Hua University, Hsinchu, Taiwan.
Appl Microbiol Biotechnol. 2002 Feb;58(2):175-7. doi: 10.1007/s00253-001-0827-1.
Cultivation of Bacillus thuringiensis for thuringiensin production is a mixed-growth-associated system. Cultivation conditions should be different during the cell growth stage and production stage. In this study, agitation speed and aeration rate were varied during the exponential growth phase and stationary phase in order to investigate the effect of shear stress via agitation on cultivation of B. thuringiensis for thuringiensin production. It was found that shear stress had a significant effect on thuringiensin production during the stationary phase. By decreasing the agitation speed during the stationary phase, product formation was increased up to 43%.
为生产苏云金素而培养苏云金芽孢杆菌是一个与混合生长相关的系统。在细胞生长阶段和生产阶段，培养条件应有所不同。在本研究中，在指数生长期和稳定期改变搅拌速度和通气速率，以研究搅拌产生的剪切应力对生产苏云金素的苏云金芽孢杆菌培养的影响。结果发现，剪切应力在稳定期对苏云金素的生产有显著影响。通过在稳定期降低搅拌速度，产物形成增加了43%。
