Osadchaia A I, Prokopchenko S F, Kostiuchenko I L, Mikhnovskaia N D, Vasilevskaia I A
Mikrobiol Zh (1978). 1990 Nov-Dec;52(6):34-9.
Optimal conditions of aeration with depth regime of cultivation of Bacillus thuringiensis H14 266/2-1, their effect on the output of biomass, endotoxin and activity of bacteria have been studied. The aeration conditions are optimized using the mathematical method of experiment planning. It is established that the air supply favours an increase of the bacillary biomass and insecticide activity for the whole cultivation period. In the first day of fermentation it is inexpedient to increase to rate of the medium agitation. To obtain a high titre of cells of the studied bacilli and to retain their activity a specific air consumption is recommended amounting to 0.5 1/1/min for the first 12h with an increase to 1.01/1/min in the following hours of fermentation, the constant rate of the medium agitation being 500 rev/min.
研究了苏云金芽孢杆菌H14 266/2-1培养深度体系下的最佳通气条件,及其对生物量产量、内毒素和细菌活性的影响。采用实验规划的数学方法优化通气条件。结果表明,在整个培养期间,通气有利于芽孢杆菌生物量和杀虫剂活性的增加。在发酵的第一天,提高培养基搅拌速率是不合适的。为了获得高滴度的所研究芽孢杆菌细胞并保持其活性,建议在最初12小时内特定空气消耗量为0.5升/升/分钟,在随后的发酵时间内增加到1.0升/升/分钟,培养基搅拌的恒定速率为500转/分钟。