The incorporation of radioactive uridine into the hepatitis B antigen of a chimpanzee.
作者信息
Jóźwiak W, Desmyter J, O'Connell A, Mortelmans J, Millman I
出版信息
Proc Soc Exp Biol Med. 1975 Oct;150(1):121-5. doi: 10.3181/00379727-150-38986.
PMID:1187682
Abstract
Radioactive (3H) uridine was incorporated into RNA isolated from the HBsAg of a chimpanzee carrier. HBsAg was purified by precipitation as an immune complex with the IgG fraction of chimpanzee anti-HBs. RNA was extracted from the washed complex with buffered phenol precipitated with alcohol and four nucleotides were identified by thin layer chromatography after alkali degradation.
摘要
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