Takahashi Yuichi, Ohashi Takeshi
Yamagata Prefectural Institute of Public Health.
Arerugi. 2002 Jan;51(1):24-9.
To examine the possibility of real-time measurement system for the concentration of airborne pollen allergens, Cry j 1 was chosen as an example to establish the system. Airborne pollen allergens were collected using Cyclone sampler from Burkard Co. Ltd. England. Cry j 1 was extracted from airborne samples with 10 mM HEPES buffer containing 0.125 M NH4HCO3 and measured with Biacore 3000 and Biacore Q. The sensitivity of the system was 5 ng/ml, and 0.1 ml sample volume and at least 500 pg of Cry j 1 was required for each measurement. Amounts of Cry j 1 can be obtained ca. 40 min after collection, that is, 15 min for extraction, 10 min for separation and 10 min for measurement.
为了研究空气中花粉过敏原浓度实时测量系统的可能性，以Cry j 1为例建立该系统。使用英国Burkard有限公司的气旋采样器收集空气中的花粉过敏原。用含有0.125 M NH4HCO3的10 mM HEPES缓冲液从空气样本中提取Cry j 1，并使用Biacore 3000和Biacore Q进行测量。该系统的灵敏度为5 ng/ml，每次测量需要0.1 ml样本体积且至少500 pg的Cry j 1。收集后约40分钟可获得Cry j 1的量，即提取15分钟、分离10分钟和测量10分钟。
