Linton Jina Lee, Sohn Byung-Wha, Yook Jong-In, Le Geros Racquel Z
Department of Orthodontics, College of Dentistry, Yonsei University, Seoul, South Korea.
Cleft Palate Craniofac J. 2002 Mar;39(2):197-207. doi: 10.1597/1545-1569_2002_039_0197_eocpcb_2.0.co_2.
The aim of this study was to evaluate the use of calcium phosphate ceramic (CPC) materials as a potential alternative to autogenous secondary alveolar bone grafting in cleft lip and palate patients who are in mixed dentition.
Four 12-week-old beagles and one 15-week-old beagle were used as subjects.
In each experimental beagle, the third and fourth deciduous premolars were extracted. The sockets were filled with four different CPC materials and sutured. MAIN OUTCOME MEASURES The beagles were fed a soft diet for the following 8 weeks and then sacrificed for clinical, radiological, histological, transmission electron microscope (TEM), and infrared (IR) absorption analysis.
All four experimental graft materials allowed normal development and eruption of permanent premolars. In histological sections, small particles of biphasic calcium phosphate (BCP) and carbonate apatite (CO(3)-AP) were resorbed, and large particulate forms served as bone frames in cortical bones. Polymer coated with carbonate apatite (Poly/CO(3)-AP) did not cause inflammation but was pushed away to the soft tissue by erupting teeth. Alginate coated with carbonate apatite (alginate/CO(3)-AP) caused a severe inflammatory reaction to the point of destroying a part of the dental follicle and cortical bone. In TEM, resorption activity by phagocytic cells was observed only in CO(3)-AP. Direct bonding of CO(3)-AP to the bone was observed as the electron-dense interface between bone and CO(3)-AP.
BCP and CO(3)-AP proved to be suitable as alveolar bone graft materials in areas where tooth eruption occurs. Of the four materials tested, CO(3)-AP produced the best results.
本研究旨在评估磷酸钙陶瓷（CPC）材料作为唇腭裂混合牙列患者自体二次牙槽骨移植潜在替代物的应用。
选用4只12周龄的比格犬和1只15周龄的比格犬作为研究对象。
在每只实验比格犬中，拔除第三和第四乳前磨牙。牙槽窝用四种不同的CPC材料填充并缝合。主要观察指标：在接下来的8周内，给比格犬喂食软食，然后处死进行临床、放射学、组织学、透射电子显微镜（TEM）和红外（IR）吸收分析。
所有四种实验性移植材料均能使恒前磨牙正常发育和萌出。在组织学切片中，双相磷酸钙（BCP）和碳酸磷灰石（CO(3)-AP）的小颗粒被吸收，大颗粒形式在皮质骨中作为骨框架。碳酸磷灰石涂层聚合物（Poly/CO(3)-AP）未引起炎症，但被萌出的牙齿推至软组织。碳酸磷灰石涂层藻酸盐（藻酸盐/CO(3)-AP）引起严重的炎症反应，以至于破坏了部分牙囊和皮质骨。在TEM中，仅在CO(3)-AP中观察到吞噬细胞的吸收活性。观察到CO(3)-AP与骨的直接结合，表现为骨与CO(3)-AP之间的电子致密界面。
BCP和CO(3)-AP被证明适合作为有牙齿萌出区域的牙槽骨移植材料。在测试的四种材料中，CO(3)-AP产生了最佳效果。
