Vacher Claire-Marie, Frétier Philippe, Créminon Christophe, Calas André, Hardin-Pouzet Hélène
Laboratory of Neurobiology of Intercellular Signals, Unité Mixte de Recherche 7101, Centre National de la Recherche Scientifique, Pierre and Marie Curie University, 75252 Paris Cedex 05, France.
J Neurosci. 2002 Mar 1;22(5):1513-22. doi: 10.1523/JNEUROSCI.22-05-01513.2002.
Noradrenaline and serotonin are known to control arginine-vasopressin (AVP) and oxytocin (OT) secretion in the systemic circulation. The aim of the current study was to investigate whether these monoamines are also able to influence AVP and OT expression in the paraventricular (PVN) and supraoptic nuclei (SON). To test this hypothesis, we used the Tg8 transgenic mice KO for the monoamine oxidase-A gene, which present high levels of noradrenaline and serotonin in the brain. AVP and OT expression were evaluated at peptide and mRNA levels by immunohistochemistry, enzyme immunoassay, and in situ hybridization. Compared with wild type, the amounts of AVP, OT, AVP mRNA, and OT mRNA were increased in the PVN and SON in Tg8 mice. To distinguish the respective contributions of noradrenaline and serotonin to these modifications, we treated Tg8 mice with a synthesis inhibitor of either catecholamines [alpha-methylparatyrosine (alpha-MPT)] or serotonin [parachlorophenylalanine (pCPA)]. Administration of alpha-MPT to Tg8 mice induced a decline in the amounts of AVP, OT, and their mRNA in the PVN and SON. The pCPA treatment in Tg8 mice was also associated with a decrease in OT expression in the PVN and SON and in AVP expression in the PVN, but not in the SON. These results suggest that noradrenaline may activate AVP and OT expression in the PVN and SON. Likewise, serotonin is proposed to stimulate AVP and OT expression in the PVN and only OT expression in the SON.
已知去甲肾上腺素和血清素可控制全身循环中精氨酸加压素（AVP）和催产素（OT）的分泌。本研究的目的是调查这些单胺是否也能够影响室旁核（PVN）和视上核（SON）中AVP和OT的表达。为了验证这一假设，我们使用了单胺氧化酶-A基因敲除的Tg8转基因小鼠，这些小鼠大脑中去甲肾上腺素和血清素水平较高。通过免疫组织化学、酶免疫测定和原位杂交在肽和mRNA水平评估AVP和OT的表达。与野生型相比，Tg8小鼠PVN和SON中AVP、OT、AVP mRNA和OT mRNA的量增加。为了区分去甲肾上腺素和血清素对这些变化的各自作用，我们用儿茶酚胺合成抑制剂[α-甲基对酪氨酸（α-MPT）]或血清素[对氯苯丙氨酸（pCPA）]处理Tg8小鼠。给Tg8小鼠注射α-MPT导致PVN和SON中AVP、OT及其mRNA的量下降。Tg8小鼠的pCPA处理还与PVN和SON中OT表达的降低以及PVN中AVP表达的降低有关，但SON中AVP表达未降低。这些结果表明，去甲肾上腺素可能激活PVN和SON中AVP和OT的表达。同样，血清素被认为可刺激PVN中AVP和OT的表达以及SON中仅OT的表达。
