Gragnani A, Morgan J R, Ferreira L M
Federal University of São Paulo, Brazil.
J Burn Care Rehabil. 2002 Mar-Apr;23(2):126-31. doi: 10.1097/00004630-200203000-00009.
The differentiation and barrier formation of cultured composite skin grafts (CSGs) were assessed by histology and measurements of surface electrical capacitance (SEC) in vitro and in vivo. Keratinocytes cultured on the surface of acellular dermis were lifted to the air-liquid interface and analyzed for 30 days in vitro. Initially, SEC measurements of CSGs (n = 11) were high but quickly dropped between days 4 and 6 and remained steady for 30 days, indicating barrier formation by the epidermis. Histology of the CSGs (n = 6) demonstrated stratification of the epidermal cells and partial formation of the stratum corneum by day 3 that was complete by day 7. CSGs (n = 5) were transplanted to athymic mice, where they formed a stratified and differentiated epidermis. SEC measurements of CSGs remained low after transplant, suggesting that exposure to the air-liquid interface improved the maturation of CSGs in vitro prior to transplant.
通过组织学和表面电容（SEC）测量，在体外和体内评估了培养的复合皮肤移植物（CSG）的分化和屏障形成。将培养在脱细胞真皮表面的角质形成细胞提升至气液界面，并在体外分析30天。最初，CSG（n = 11）的SEC测量值较高，但在第4天至第6天之间迅速下降，并在30天内保持稳定，表明表皮形成了屏障。CSG（n = 6）的组织学显示，到第3天表皮细胞分层，角质层部分形成，到第7天完全形成。将CSG（n = 5）移植到无胸腺小鼠体内，它们在那里形成了分层且分化的表皮。移植后CSG的SEC测量值仍然很低，这表明在移植前暴露于气液界面可改善CSG在体外的成熟度。
