Sandaa R A, Heldal M, Castberg T, Thyrhaug R, Bratbak G
Department of Microbiology, University of Bergen, Postbox 7800, N-5020, Bergen, Norway.
Virology. 2001 Nov 25;290(2):272-80. doi: 10.1006/viro.2001.1161.
Two lytic viruses specific for Chrysochromulina ericina (Prymnesiophyceae) and for Pyramimonas orientalis (Prasinophyceae) were isolated from Norwegian coastal waters in June 1998. The lytic cycle was 14-19 h for both viruses; the burst size was estimated at 1800-4100 viruses per host cell for the Chrysochromulina virus and 800-1000 for the Pyramimonas virus. Thin sections of infected cells show that both viruses replicate in the cytoplasm and that they have a hexagonal cross section, indicating icosahedral symmetry. The Chrysochromulina virus had a particle size of 160 nm and a genome size of 510 kbp; the size of the major polypeptide was 73 kDa. The Pyramimonas virus had a particle size of 220 x 180 nm and a genome size of 560 kbp; the size of the major polypeptide was 44 kDa. The genome sizes of these viruses are among the largest ever reported for viruses and they are larger than the minimum required for cellular life. The Chrysochromulina virus clone CeV-01B and the Pyramimonas virus clone PoV-01B described in this study have several properties in common with other viruses infecting microalgae, suggesting that they belong to the Phycodnaviridae.
1998年6月，从挪威沿海水域分离出两种分别特异性感染埃里克金色藻（定鞭藻纲）和东方扁藻（绿藻纲）的裂解性病毒。两种病毒的裂解周期均为14 - 19小时；金色藻病毒的裂解量估计为每个宿主细胞产生1800 - 4100个病毒，扁藻病毒为800 - 1000个。受感染细胞的超薄切片显示，两种病毒均在细胞质中复制，且具有六边形横截面，表明为二十面体对称。金色藻病毒的颗粒大小为160纳米，基因组大小为510千碱基对；主要多肽大小为73千道尔顿。扁藻病毒的颗粒大小为220×180纳米，基因组大小为560千碱基对；主要多肽大小为44千道尔顿。这些病毒的基因组大小属于病毒中报道过的最大之列，且大于细胞生命所需的最小值。本研究中描述的金色藻病毒克隆CeV - 01B和扁藻病毒克隆PoV - 01B与其他感染微藻的病毒有若干共同特性，表明它们属于藻病毒科。
