Gajigan Andrian P, Schvarcz Christopher R, Conaco Cecilia, Edwards Kyle F, Steward Grieg F
Department of Oceanography, University of Hawai'i at Mānoa, Honolulu, HI, USA.
Center for Microbial Oceanography Research and Education, University of Hawai'i at Mānoa, Honolulu, HI, USA.
Npj Viruses. 2025 May 31;3(1):47. doi: 10.1038/s44298-025-00128-7.
The genome of Oceanusvirus kaneohense isolate, Tetraselmis virus 1 (TetV-1), was previously sequenced, but little was known about its infection cycle. We determined the eclipse period (4-8 h), latent period (16 h), and burst size (~1000), and observed ultrastructural changes in the host during infection. Putative viral factories and inclusion bodies appear in the cytoplasm by 8 and 16 h post-infection, respectively. Of 830 viral transcripts detected, those related to transcription, DNA synthesis, and host immune repression appeared earlier (0.25 and 4 h), and virus structural genes appeared later (8-16 h). Host lipid metabolism and endocytosis-related transcripts were upregulated during the early phase, while protein modification genes were downregulated. In the later stages, host transcripts associated with basic cellular processes were relatively more common, while developmental genes became less so. Many highly expressed host and virus genes were of unknown function, highlighting a need for additional functional studies.
海洋卡内奥赫病毒分离株四爿藻病毒1（TetV-1）的基因组此前已被测序，但对其感染周期了解甚少。我们确定了隐蔽期（4-8小时）、潜伏期（16小时）和裂解量（约1000），并观察了感染期间宿主的超微结构变化。假定的病毒工厂和包涵体分别在感染后8小时和16小时出现在细胞质中。在检测到的830个病毒转录本中，与转录、DNA合成和宿主免疫抑制相关的转录本出现较早（0.25小时和4小时），而病毒结构基因出现较晚（8-16小时）。宿主脂质代谢和内吞作用相关的转录本在早期上调，而蛋白质修饰基因下调。在后期，与基本细胞过程相关的宿主转录本相对更常见，而发育基因则不然。许多高表达的宿主和病毒基因功能未知，这突出表明需要进行更多的功能研究。
