Songsri P, Hamazaki T, Ishikawa Y, Yamada T
Department of Fermentation Technology, Faculty of Engineering, Hiroshima University, Japan.
Virology. 1995 Dec 20;214(2):405-12. doi: 10.1006/viro.1995.0050.
Large (30-45 kbp) deletions were induced in the Chlorella virus CVK1 genome by UV irradiation. Restriction endonuclease maps of the mutant genomes showed that these deletions occurred in a region located from 1.5 kbp to 47 kbp from the left DNA end. The nucleotide sequences determined around the deletion boundaries indicate that the deletion process took place by both homologous and nonhomologous recombinations. In one case, the recombination site was within a region of about 600 bp, consisting of 40 tandem repetitions of a 15-bp sequence element. The deleted region may contain several multigene families. Northern blot analyses with probes including the genes for translational elongation factor 3 and DNA polymerase showed no discernible aberrancy in the gene expression patterns in the mutants. However, at least two protein bands were missing from the mutant virions.
通过紫外线照射在小球藻病毒CVK1基因组中诱导产生了大片段（30 - 45千碱基对）的缺失。突变基因组的限制性内切酶图谱显示，这些缺失发生在距离DNA左端1.5千碱基对至47千碱基对的区域。在缺失边界周围测定的核苷酸序列表明，缺失过程是通过同源重组和非同源重组共同发生的。在一个案例中，重组位点位于一个约600碱基对的区域内，该区域由一个15碱基对序列元件的40个串联重复组成。缺失区域可能包含几个多基因家族。用包括翻译延伸因子3和DNA聚合酶基因的探针进行的Northern印迹分析表明，突变体中的基因表达模式没有明显异常。然而，突变体病毒粒子中至少缺少两条蛋白带。
