Brussaard C P D, Noordeloos A A M, Sandaa R-A, Heldal M, Bratbak G
Department of Biological Oceanography, Royal Netherlands Institute for Sea Research, NL-1790 AB Den Burg, The Netherlands.
Virology. 2004 Feb 20;319(2):280-91. doi: 10.1016/j.virol.2003.10.033.
We report the isolation of the first double-stranded (ds) RNA virus in the family Reoviridae that infects a protist (microalga Micromonas pusilla, Prasinophyceae). The dsRNA genome was composed of 11 segments ranging between 0.8 and 5.8 kb, with a total size of approximately 25.5 kb. The virus (MpRNAV-01B) could not be assigned to the genus level because host type, genome size, and number of segments smaller than 2 kb did not correspond to either of the two existing 11-segmented dsRNA genera Rotavirus and Aquareovirus. MpRNAV-01B has a particle size of 65-80 nm, a narrow host range, a latent period of 36 h, and contains five major proteins (120, 95, 67, 53, and 32 kDa). MpRNAV-01B was stable to freeze-thawing, resistant to chloroform, ether, nonionic detergents, chelating and reducing agents. The virus was inactivated at temperatures above 35 degrees C and by ionic detergent, ethanol, acetone, and acidic conditions (pH 2-5).
我们报告了呼肠孤病毒科中首个感染原生生物（绿藻微小单胞藻，绿藻门）的双链（ds）RNA病毒的分离情况。该dsRNA基因组由11个片段组成，长度在0.8至5.8 kb之间，总大小约为25.5 kb。该病毒（MpRNAV - 01B）无法归类到属一级，因为宿主类型、基因组大小以及小于2 kb的片段数量均与现有的两个拥有11个片段的dsRNA属——轮状病毒属和水生呼肠孤病毒属不符。MpRNAV - 01B的颗粒大小为65 - 80 nm，宿主范围狭窄，潜伏期为36小时，包含五种主要蛋白（120、95、67、53和32 kDa）。MpRNAV - 01B对冻融稳定，对氯仿、乙醚、非离子去污剂、螯合剂和还原剂有抗性。该病毒在35摄氏度以上的温度以及离子去污剂、乙醇、丙酮和酸性条件（pH 2 - 5）下会失活。
