Nowak M, Szpakowski M, Malinowski A, Wieczorek A, Maciołek-Blewniewska G, Wilczyński J R, Szpakowski A, Wierzbicka E, Małafiej E, Oszukowski P
Kliniki Chirurgii Ginekologicznej Instytutu Centrum Zdrowia Matki Polki w Łodzi.
Ginekol Pol. 2001 Dec;72(12A):1444-8.
The aim of our study was to evaluate serum interleukin-6 (IL-6), interleukin-8 (IL-8), tumor necrosis factor-alpha (TNF-alpha) and interferon-gamma (IFN-gamma) as tumor markers--study based on the data about tumor cytokines production and tumor-host interactions.
We investigated 48 women: 17 with ovarian cancer untreated before, 16 with benign ovarian cysts and 15 healthy controls. Venous blood for cytokines determinations were obtained before operations and during routine screening tests. Titers of cytokines were measured by means of ELISA technique.
In the control group the upper limit of normal IL-6 titers (95th percentile) was 5.5 pg/ml; the mean IL-8 concentration was 9.6 +/- 15.1 pg/ml and the upper limit of normal was 37 pg/ml; serum TNF-alpha and IFN-gamma were not detectable. In patients with benign ovarian cysts the levels of all investigated cytokines didn't differ significantly from healthy controls. Women with ovarian cancer had significantly higher serum IL-8 levels (mean: 290.5 +/- 351 pg/ml) than healthy controls or women with benign ovarian cysts; 88% of them had IL-8 titers above the normal. The IL-6 titers in ovarian cancer were also higher but didn't reach statistical significance, 53% of them had IL-8 titers above the normal. TNF-alpha and IFN-gamma levels in ovarian cancer were similar to patients with benign ovarian cysts.
Serum IL-8 levels in patients with ovarian cancer were significantly higher when compared to healthy controls and benign ovarian cysts and in almost 90% of ovarian cancers the titers of IL-8 were increased. Additionally, 53% of women with ovarian cancer had elevated serum IL-6 levels.
我们研究的目的是评估血清白细胞介素-6（IL-6）、白细胞介素-8（IL-8）、肿瘤坏死因子-α（TNF-α）和干扰素-γ（IFN-γ）作为肿瘤标志物——基于肿瘤细胞因子产生及肿瘤与宿主相互作用的数据进行的研究。
我们调查了48名女性：17名未经治疗的卵巢癌患者、16名患有良性卵巢囊肿的女性和15名健康对照者。在手术前和常规筛查测试期间采集用于细胞因子测定的静脉血。通过ELISA技术测量细胞因子的滴度。
在对照组中，IL-6滴度的正常上限（第95百分位数）为5.5 pg/ml；IL-8的平均浓度为9.6±15.1 pg/ml，正常上限为37 pg/ml；血清TNF-α和IFN-γ未检测到。患有良性卵巢囊肿的患者中，所有研究的细胞因子水平与健康对照者无显著差异。卵巢癌女性的血清IL-8水平（平均：290.5±351 pg/ml）显著高于健康对照者或患有良性卵巢囊肿的女性；其中88%的患者IL-8滴度高于正常水平。卵巢癌患者的IL-6滴度也较高，但未达到统计学显著性，其中53%的患者IL-8滴度高于正常水平。卵巢癌患者的TNF-α和IFN-γ水平与患有良性卵巢囊肿的患者相似。
与健康对照者和良性卵巢囊肿患者相比，卵巢癌患者的血清IL-8水平显著更高，且在几乎90%的卵巢癌患者中IL-8滴度升高。此外，53%的卵巢癌女性血清IL-6水平升高。
