Carmona Pedro, Molina Marina
Instituto de Estructura de la Materia (CSIC), Serrano 121, 28006 Madrid, Spain.
Nucleic Acids Res. 2002 Mar 15;30(6):1333-7. doi: 10.1093/nar/30.6.1333.
Infrared and UV spectroscopies have been used to study the assembly of a hairpin nucleotide sequence (nucleotides 3-30) of the 5' non-coding region of the hepatitis C virus RNA (5'-GGCGGGGAUUAUCCCCGCUGUGAGGCGG-3') with a RNA 20mer ligand (5'-CCGCCUCACAAAGGUGGGGU-3') in the presence of magnesium ion and spermidine. The resulting complex involves two helical structural domains: the first one is an intermolecular duplex stem at the bottom of the target hairpin and the second one is a parallel triplex generated by the intramolecular hairpin duplex and the ligand. Infrared spectroscopy shows that N-type sugars are exclusively present in the complex. This is the first case of formation of a RNA parallel triplex with purine motif and shows that this type of targeting RNA strands to viral RNA duplexes can be used as an alternative to antisense oligonucleotides or ribozymes.
在镁离子和亚精胺存在的情况下,利用红外光谱和紫外光谱研究了丙型肝炎病毒RNA 5'非编码区(5'-GGCGGGGAUUAUCCCCGCUGUGAGGCGG-3')的发夹核苷酸序列(核苷酸3 - 30)与RNA 20聚体配体(5'-CCGCCUCACAAAGGUGGGGU-3')的组装。形成的复合物包含两个螺旋结构域:第一个是靶发夹底部的分子间双链茎,第二个是由分子内发夹双链和配体产生的平行三链体。红外光谱表明该复合物中只存在N型糖。这是首次形成具有嘌呤基序的RNA平行三链体的情况,表明这种将RNA链靶向病毒RNA双链体的方式可作为反义寡核苷酸或核酶的替代方法。