利用细菌几丁质酶从结晶几丁质定量生产2-乙酰氨基-2-脱氧-D-葡萄糖。
Quantitative production of 2-acetamido-2-deoxy-D-glucose from crystalline chitin by bacterial chitinase.
作者信息
Pichyangkura Rath, Kudan Sanya, Kuttiyawong Kamontip, Sukwattanasinitt Mongkol, Aiba Sei-ichi
机构信息
Department of Biochemistry, Faculty of Science, Chulalongkorn University, Bangkok, Thailand.
出版信息
Carbohydr Res. 2002 Mar 15;337(6):557-9. doi: 10.1016/s0008-6215(02)00007-1.
Finely powdered alpha- and beta-chitin can be completely hydrolyzed with chitinase (EC 3.2.1.14) and beta-N-acetylhexosaminidase (EC 3.2.1.52) for the production of 2-acetamido-2-deoxy-D-glucose (GlcNAc). Crude chitinase from Burkholderia cepacia TU09 and Bacillus licheniformis SK-1 were used to digest alpha- and beta-chitin powder. Chitinase from B. cepacia TU09 produced GlcNAc in greater than 85% yield from beta- and alpha-chitin within 1 and 7 days, respectively. B. licheniformis SK-1 chitinase completely hydrolyzed beta-chitin within 6 days, giving a final GlcNAc yield of 75%, along with 20% of chitobiose. However, only a 41% yield of GlcNAc was achieved from digesting alpha-chitin with B. licheniformis SK-1 chitinase.
精细粉末状的α-和β-几丁质可以用几丁质酶（EC 3.2.1.14）和β-N-乙酰己糖胺酶（EC 3.2.1.52）完全水解，以生产2-乙酰氨基-2-脱氧-D-葡萄糖（GlcNAc）。来自洋葱伯克霍尔德菌TU09和地衣芽孢杆菌SK-1的粗几丁质酶被用于消化α-和β-几丁质粉末。来自洋葱伯克霍尔德菌TU09的几丁质酶分别在1天和7天内从β-和α-几丁质中产生了产率大于85%的GlcNAc。地衣芽孢杆菌SK-1几丁质酶在6天内完全水解了β-几丁质，最终GlcNAc产率为75%，同时还有20%的壳二糖。然而，用地衣芽孢杆菌SK-1几丁质酶消化α-几丁质时，GlcNAc的产率仅为41%。
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