Cardozo Flávio Augusto, Gonzalez Juan Miguel, Feitosa Valker Araujo, Pessoa Adalberto, Rivera Irma Nelly Gutierrez
Department of Microbiology, Biomedical Sciences Institute, University of São Paulo, 1374 Professor Lineu Prestes Ave., São Paulo, 05508-000, Brazil.
Institute of Natural Resources and Agrobiology of Seville, Spanish National Research Council, 10 Reina Mercedes Ave., 41012, Seville, Spain.
World J Microbiol Biotechnol. 2017 Oct 27;33(11):201. doi: 10.1007/s11274-017-2373-8.
N-Acetyl-D-glucosamine (GlcNAc) is a monosaccharide with great application potential in the food, cosmetic, pharmaceutical, and biomaterial areas. GlcNAc is currently produced by chemical hydrolysis of chitin, but the current processes are environmentally unfriendly, have low yield and high cost. This study demonstrates the potential to produce GlcNAc from α-chitin using chitinases of ten marine-derived Aeromonas isolates as a sustainable alternative to the current chemical process. The isolates were characterized as Aeromonas caviae by multilocus sequence analysis (MLSA) using six housekeeping genes (gltA, groL, gyrB, metG, ppsA, and recA), not presented the virulence genes verified (alt, act, ast, ahh1, aer, aerA, hlyA, ascV and ascFG), but showed hemolytic activity on blood agar. GlcNAc was produced at 37 °C, pH 5.0, 2% (w/v) colloidal chitin and crude chitinase extracts (0.5 U mL) by all the isolates with yields from 14 to 85% at 6 h, 17-89% at 12 h and 19-93% after 24 h. The highest yield of GlcNAc was observed by A. caviae CH129 (93%). This study demonstrates one of the most efficient chitin enzymatic hydrolysis procedures and A. caviae isolates with great potential for chitinases expression and GlcNAc production.
N-乙酰-D-葡萄糖胺（GlcNAc）是一种单糖，在食品、化妆品、制药和生物材料领域具有巨大的应用潜力。目前，GlcNAc是通过几丁质的化学水解生产的，但当前工艺对环境不友好，产量低且成本高。本研究表明，利用十种海洋来源气单胞菌分离株的几丁质酶从α-几丁质生产GlcNAc具有潜力，可作为当前化学工艺的可持续替代方法。通过使用六个管家基因（gltA、groL、gyrB、metG、ppsA和recA）的多位点序列分析（MLSA），将这些分离株鉴定为豚鼠气单胞菌，未检测到已验证的毒力基因（alt、act、ast、ahh1、aer、aerA、hlyA、ascV和ascFG），但在血琼脂上显示出溶血活性。所有分离株在37℃、pH 5.0、2%（w/v）胶体几丁质和粗几丁质酶提取物（0.5 U/mL）的条件下生产GlcNAc，6小时时产量为14%至85%，12小时时为17%至89%，24小时后为19%至93%。豚鼠气单胞菌CH129观察到GlcNAc的最高产量（93%）。本研究证明了最有效的几丁质酶促水解程序之一，以及豚鼠气单胞菌分离株在几丁质酶表达和GlcNAc生产方面具有巨大潜力。
