Sato Kiichi, Yamanaka Maho, Takahashi Hiroko, Tokeshi Manabu, Kimura Hiroko, Kitamori Takehiko
Department of Applied Chemistry, School of Engineering, The University of Tokyo, Tokyo, Japan.
Electrophoresis. 2002 Mar;23(5):734-9. doi: 10.1002/1522-2683(200203)23:5<734::AID-ELPS734>3.0.CO;2-W.
A bead-bed immunoassay system suitable for simultaneous assay of multiple samples was constructed on a microchip. The chip had branching multichannels and four reaction and detection regions; the constructed system could process four samples at a time with only one pump unit. Interferon gamma was assayed by a 3-step sandwich immunoassay with the system coupled to a thermal lens microscope as a detector. The biases of the signal intensities obtained from each channel were within 10%, and coefficients of variation were almost the same level as the single straight channel assay. The assay time for four samples was 50 min instead of 35 min for one sample in the single-channel assay; hence higher throughput was realized with the branching structure chip.
在微芯片上构建了一种适用于同时检测多个样品的珠床免疫分析系统。该芯片具有分支多通道和四个反应及检测区域；构建的系统仅用一个泵单元就能一次处理四个样品。使用该系统与热透镜显微镜作为检测器，通过三步夹心免疫分析法检测干扰素γ。从每个通道获得的信号强度偏差在10%以内，变异系数与单一直通道分析几乎处于同一水平。单通道分析中一个样品的检测时间为35分钟，而四个样品的检测时间为50分钟；因此，使用分支结构芯片实现了更高的通量。
