Sato Kiichi, Yamanaka Maho, Hagino Tomokazu, Tokeshi Manabu, Kimura Hiroko, Kitamori Takehiko
Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Japan.
Lab Chip. 2004 Dec;4(6):570-5. doi: 10.1039/b411356j. Epub 2004 Nov 10.
A microchip-based enzyme-linked immunosorbent assay (microELISA) system was developed and interferon-gamma was successfully determined. The system was composed of a microchip with a Y-shaped microchannel and a dam structure, polystyrene microbeads, and a thermal lens microscope (TLM). All reactions required for the immunoassay were done in the microchannel by successive introduction of a sample and regents. The enzyme reaction product, in a liquid phase, was detected downstream in the channel using the TLM as substrate solution was injected. The antigen-antibody reaction time was shortened by the microchip integration. The limit of the determination was improved by adopting the enzyme label. Moreover, detection procedures were greatly simplified and required time for the detection was significantly cut. The system has good potential to be developed as a small and automated high throughput analyzer.
开发了一种基于微芯片的酶联免疫吸附测定（微ELISA）系统，并成功测定了干扰素-γ。该系统由具有Y形微通道和坝结构的微芯片、聚苯乙烯微珠和热透镜显微镜（TLM）组成。免疫测定所需的所有反应通过连续引入样品和试剂在微通道中进行。当注入底物溶液时，使用TLM在通道下游检测液相中的酶反应产物。微芯片集成缩短了抗原-抗体反应时间。采用酶标记提高了测定限。此外，检测程序大大简化，检测所需时间显著缩短。该系统具有开发成为小型自动化高通量分析仪的良好潜力。
